过量表达pAPX基因提高水稻对镉胁迫的耐性  

OVEREXPRESSING PEROXISOMAL ASCORBATE PEROXIDASE GENE IN RICE ENHANCED TOLERANCE TO CADMIUM STRESS

作　　者：段苏然[1] 施卫明[2] 王俊儒[1] 

机构地区：[1]西北农林科技大学生命科学学院,陕西杨凌712100 [2]土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008

出　　处：《土壤学报》2006年第1期111-116,共6页Acta Pedologica Sinica

基　　金：国家自然科学基金项目(40271063)资助

摘　　要：研究了编码大麦pAPX的基因HvAPX1导入水稻并过量表达后,转pAPX水稻在镉胁迫下的生长状况、生理指标及镉含量。结果发现,由于pAPX基因的过量表达,转基因植株的根系伸长量、生物量、叶绿素含量以及APX活性都明显高于野生型植株。与野生型植株相比,转基因水稻对镉胁迫具有明显的耐性。伴随对镉胁迫耐性的提高,转pAPX水稻对镉的累积量也同时提高。Differences were studied between wild type and transgenic rice overexpressing barley peroxisomal type ascorbate peroxidase （ HvAPX1 ） under excessive cadmium condition in growth, physiological characters and accumulation of cadmium. The results indicate that with increasing HvAPXI transcript in transgenic rice, elongation rate of the main root, total biomass, chlorophyll content and activity of the APX （ascorbate peroxidase） all got obviously higher than that of the wild type of rice, respectively. The transgenic plants were significantly more tolerant to cadmium stress as compared with the wide-type plants and accumulate more cadmium.

关 键 词：镉胁迫 抗坏血酸过氧化物酶 环境胁迫 基因过量表达 转基因水稻 

分 类 号：S154.4[农业科学—土壤学]