恶臭假单胞菌(Pseudomonas putida)GM6的聚磷特性研究  

CHARACTERIZATION OF PSEUDOMONAS PUTIDA GM6 WITH HIGH CAPABILITY OF ACCUMULATING POLY-P

作　　者：蔡天明[1] 管莉菠[1] 崔中利[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院微生物系南京农业大学农业部环境微生物重点开放实验室,南京210095

出　　处：《土壤学报》2006年第1期117-123,共7页Acta Pedologica Sinica

基　　金：江苏省社会发展项目(BS2003028);江苏省环境保护厅项目(2004007)资助

摘　　要：从城市污水处理厂好氧池活性污泥中分离获得一株高效聚磷菌株GM6，经生理生化和16SrDNA初步鉴定为恶臭假单胞菌（Pseudomonas putida）。GM6生长pH在5．5至8．5之间，最适生长pH为6．5；当pH值为7．0时，聚磷能力最强，pH值小于5．5或大于7．5时，除磷能力明显下降。通气量试验表明，装液量对GM6的生长影响较小；装液量为100ml时，磷的去除效果最好，当装液量大于150ml时磷的去除效果变差。GM6的最适生长温度为27℃，当温度小于5℃或大于37℃时生长较慢；当温度为20℃其除磷效果最好，温度大于33℃或小于5℃时，除磷效果明显变差。好氧条件下GM6在合成废水、MOPS培养基、LB及YG培养基中培养时磷的绝对去除量分别为9．87、12．1、87．3和67．1mgL^-1，磷的去除率分别为96．6％、85％、71．6％和63％，磷的绝对去除量和去除率远高于E．coli。好氧培养时菌体吸磷能力测定结果表明，GM6在合成废水、MOPS、LB及YG培养基中培养24h的菌体干重含磷量在6．80％～9．32％之间，而对照菌体含磷量在0．98％～2．31％之间，聚磷菌的聚磷效果远高于对照菌。用序批式反应器对GM6进行厌氧好氧纯培养时，厌氧末的上清液磷浓度为16．8mgL^-1，CODCr为230mgL^-1，放磷速率为4．5mgL^-1h^-1；好氧末的上清液磷浓度为2．56mgL^-1，CODCr为40mgL^-1，好氧吸磷速率为2．73mgL^-1h^-1，菌株GM6表现出了明显的好氧吸磷、厌氧放磷现象，具有聚磷菌的典型特征。A bacterial strain GM6 with high capability of accumulating poly-P was isolated from aerobic activated sludge in a municipal sewage treatment plant and identified as Pseudomonas putida. This strain could grow in conditions with pH ranging from 5.5 to 8.5 and the most favorably in solution being 6.5 in pH. But its capability of accumulating poly-P peaked when pH was at 7.0. When pH was either below 5.5 or above 7.5, this capability descended distinctly. Aeration tests showed that volume of liquid did not influence much growth of GM6. But when the volume was 100 million, its P removing effect was the best and while the volume is over 150 ml, the effect became less. The optimal temperature for its growth was 27℃ . When the temperature was either lower than 5 ℃ or higher than 37 ℃ , it grew slowly and its P removing effect was poor. The optimal temperature for its phosphorus removal was 20℃. After GM6 incubation in synthesized sewage, LB, YG and MOPS medium for 24 hours, phosphate concentration in the liquid media declined sharply, showing a phosphate removal rate ranging from 63 % to 96.6%, which were significantly higher than that of E. coli. Its phosphate accumulating capacity was also investigated aerobically in media LB, YG, MOPS and synthetic wastewater. The results show that the total phosphorus in the GM6 cells harvested from four media was in the range of 6.80% - 9.32% while that in the E. coli cells was in the range of 0.98% - 2.31%, indicating the former had a higher level of phosphorus accumulation. Strain DM6 showed the characteristics of a typical phosphate accumulating organism as it displayed apparent phosphorus accumulation and release in a SBR process. Its phosphate release and accumulation rate was 4.5 mg L^-1 h^-1 at the end of anaerobic stage and 2.73 mg L^-1 h^-1 at the end of subsequent aerobic stage, respectively.

关 键 词：聚磷菌 合成废水 鉴定 SBR 厌氧放磷 好氧吸磷 

分 类 号：S154.39[农业科学—土壤学]