毛细管电泳微芯片在临床尿蛋白检测中的应用研究  

Capillary Electrophoresis Microchip Assay of Clinical Urinary Proteins

作　　者：庄贵生[1] 刘菁[1] 贾春平[1] 金庆辉[1] 王惠民[2] 杨梦苏[1] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所 [2]南通大学附属医院,南通226001

出　　处：《化学学报》2006年第3期229-234,共6页Acta Chimica Sinica

基　　金：国家863计划(Nos.2004AA404252;2002AA2Z2021)资助项目

摘　　要：用微芯片毛细管电泳法对临床患者尿蛋白进行了分离,初步探讨了用于判断肾损伤的应用前景.以pH10.3,75mmol?L-1的硼酸盐缓冲液作为芯片电泳缓冲体系,利用蛋白质的紫外吸收特性,在210nm波段检测吸光度并进行信号收集和分析.研究两种添加剂对提高尿蛋白分离效率的影响,分析了肾病综合症、妊娠高血压症、风湿性心脏病和多发性骨髓瘤等患者尿样本,并与美国Helena电泳系统分析结果对比,得到了较一致的结果.The separation and detection of urinary proteins have been conducted by microchip-based capillary electrophoresis （MCE）, and application foreground of MCE in clinical diagnosis of various states of disease was discussed. Microchip-based capillary electrophoresis of urinary proteins could offer several advantages over standard urinary protein assay methods such as SDS-PAGE and conventional capillary electrophoresis, including the decrease of sample consumption and analysis time. The microchip used in these studies was designed to realize on-chip separation of urinary proteins with UV detection. The characteristic absorbency near 210 nm of proteins was adopted under the condition： the electrophoretic buffer at 75 mmol·L^-1 berate with 0.5 mol·L^-1 lactate at pH 10.5. Urine samples from four clinical cases were selected as target for microchip-based urinary protein assays, and the results were consistent with those of commercial Helena Rapid Electrophoreses system.

关 键 词：毛细管电泳微芯片 紫外检测 临床诊断 尿蛋白 

分 类 号：R446.12[医药卫生—诊断学]