温度对手性固定相HPLC分离克仑特罗和丙卡特罗的影响  

Influence of temperature on the enantioseparations of clenbuterol and procatreol on a chiral column

作　　者：孙娟[1] 唐小海 宋航[1] 唐琴[3] 付超[1] 

机构地区：[1]四川大学制药与生物工程系,四川成都610065 [2]成都药友科技发展有限公司,四川成都610041 [3]湖北民族学院化工系,湖北恩施445000

出　　处：《华西药学杂志》2006年第1期30-32,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的讨论温度对药物克仑特罗和丙卡特罗在手性固定相Whelk-O1上手性分离因子（α）的影响。方法考察在不同流动相配比条件下，柱温在-10℃℃～30℃间变化时lnα与1／T的关系，计算出两种药物对映体与固定相相互作用的焓变差值和熵变差值。结果手性分离因子α随温度增加而变小，lna与1／T呈线性关系。结论两种药物对映体的拆分过程都是焓控过程。OBJECTIVE To investigate the influence of temperature on the enantioseparations of clenbuterol and procatreol on Whelk - O1 CSP.METHODS With various mobile phase compositions, the relationship between lnα and 1/T was investigated during the column temperature changing from - 10℃ to 30℃, and the thermodynamic parameters were calculated. RESULTS The enantionseparations decreased when thetemperature increased, and the relationship between lnα and 1/T was linear. CONCLUSION These chiral recognition processes were controlled by enthalpy.

关 键 词：高效液相色谱 对映体分离 手性固定相Whelk—O1 热力学参数 克仑特罗 丙卡特罗 

分 类 号：R917[医药卫生—药物分析学]