灯盏花素脂质体的制备工艺  

Preparation process of breviscapine liposome

作　　者：罗霄[1] 宋民宪[2] 蒲旭峰 付超美[1] 周敏[1] 

机构地区：[1]成都中医药大学药学院,四川成都611730 [2]四川省食品药品监督管理局,四川成都610031 [3]成都市药品检验所,四川成都610062

出　　处：《华西药学杂志》2006年第1期54-56,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的研究灯盏花素脂质体的制备工艺。方法采用薄膜蒸发-探头超声法和冷冻干燥法制备灯盏花素脂质体,在单因素考察基础上采用正交试验设计。以包封率为评价指标,筛选脂质体制备的最佳工艺条件。冻干品水合后,在电镜下观察灯盏花素脂质体的形态,利用马尔文测定仪测定脂质体的粒径,用RP-HPLC法测定其包封率。结果灯盏花素脂质体的最佳工艺处方为药脂比1∶5,SPC∶CH为2∶1,二氯甲烷用量为10 ml。冻干保护剂蔗糖用量为10%。制备3批脂质体,包封率平均为87.5%,平均粒径为378.3 nm。结论所制脂质体包封率较高,粒径分布较均匀。OBJECTIVE To study the preparation method of breviscapine liposome. METHODS Liposomes were prepared by thin film evaporation and probe ultrasonic technique, then treated further by lyophilization. Orthagonal design was adopted to screen the preparation of breviscapine liposomes after the study of different single variables. The optimal technological conditions were acquired by the evaluation of the encapsulation efficiencies. Electronic microscopy was used to observe the shape of reconstituted liposomes. The particle sizes were determined by Zetamaster and the encapsulation efficiencies we,＇e determined by RP - HPLC. RESULTS The optimal preparation conditions of breviscapine liposome were as follows： the proportion of breviscapine and lipids in weight was 1：5; soybean phosphatidylcholine： cholesterol- 2：1, with 10 ml of dichloromethane as solvent and 10% sucrose as stabilizer of lyophilization. The average encapsulation efficiency of the optimized liposome was 87.5% and the mean particle size was 378.3 nm. CONCLUSION The entrapment efficiency and particle size of the optimized breviscapine liposome are satisfactory.

关 键 词：灯盏花素 脂质体 冷冻干燥 包封率 高效液相色谱 

分 类 号：R94[医药卫生—药剂学]