HPLC测定氯霉素滴眼液中氯霉素及二醇物的含量  

Determination of chloramphenicol and glycols in Chloramphenicol eye drops by HPLC

作　　者：谢楠[1] 何晓英[2] 张晓明[2] 郭朝晖[1] 蒋生祥[1] 

机构地区：[1]中国科学院兰州化学物理研究所甘肃省天然药物重点实验室,甘肃兰州730000 [2]甘肃省药品检验所,甘肃兰州730000

出　　处：《华西药学杂志》2006年第1期93-95,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用RP—HPLC测定氯霉素滴眼液中氯霉素及二醇物的含量。方法采用Kromasil—C18柱（4．6mm×150mm，5μm），以乙腈-水-冰醋酸（40：60：0．5）为流动相，流速1．0ml·min^-1，检测波长272nm。结果氯霉素的线性范围为8.0～128μg·ml^-1（r=0．9998），二醇物的线性范围为2．5-40．0μg·ml^-1（r=0．9998），平均回收率为98．88％，RSD=0．61％（n=6）.结论该法分离效果好，灵敏度高、重复性好、结果准确可靠。OBJECTIVE To establish an RP- HPLC method for determination of the content of chloramphenicol and glycols in Chloramphenicol eye drops. METHODS The samples were separated by Kromasil - C18 column （4.6 mm×1.50 mm,5μm） with aeetonitfile - wateracetic acid （40：60：0.5）as mobile phil, 1.0 ml·min^-1 as flow rate and detectation at 272 nm. RESULTS The calibration curve was linear in the range of 8.0 - 128.0μg·ml^-1 for determination of chloramphenicol and of 2.5 - 40.0μg·ml^-1 for glycols; the average recovery was 98.88% with RSD of 0.61% （ n =- 6）. CONCLUSION The method is good in the aspect of resolution, reproducibility and sensitivity.

关 键 词：氯霉素滴眼液 氯霉素 氯霉素二醇物 反相高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]