梅花鹿源BVDV NS_(2-3)基因重要区的克隆与序列分析  

Cloning and sequencing of NS_(2-3) of BVDV on sika deer

作　　者：温铁锋[1] 郜玉钢[1] 王树志[1] 杜锐[1] 

机构地区：[1]吉林农业大学中药材学院,吉林长春130118

出　　处：《西北农林科技大学学报（自然科学版）》2006年第1期93-96,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30300256);吉林省科技发展计划项目(20030552-2)

摘　　要：根据牛病毒性腹泻病毒(BVDV)NADL株的序列设计并合成1对引物,对从吉林不同地区分离的4株梅花鹿源BVDV(CCSYD株、CC JYD株、CCKCD株和JLCYD株)的N S2-3基因进行RT-PCR扩增,并对扩增的片段进行序列分析。结果表明,国内梅花鹿源BVDV CCSYD株属基因Ib亚型,CC JYD株、CCKCD株和JLCYD株属基因型待定。A pair of primers were designed and synthesized according to nucleotide sequence of NADL BVDV. Exogenous sequence insertion domain of NS（2-3） of CCSYD,CCTYD, CCKCD and JLCYD isolated from different districts of Jilin province was amplified by using RT PCR. The amplified fragments were cloned and sequenced. Results show that CCSYD strain of BVDV in our country belongs to subtype 1 b, but CCJYD,CCKCD,JLYD belong to some unknown gene types.

关 键 词：梅花鹿 牛病毒性腹泻病毒 NS(2-3)基因 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学]