肠内免疫营养联合重组人生长激素对烫伤大鼠的治疗作用  

Experiment study of enteral immunonutrition combined with recombinant human growth hormone in treatment of severe scald in rats

作　　者：郭光华[1] 蔡晨[2] 李国辉[1] 

机构地区：[1]南昌大学第一附属医院烧伤中心,330006 [2]安徽医科大学第一附属医院烧伤科,合肥230022

出　　处：《中国危重病急救医学》2006年第1期42-45,共4页Chinese Critical Care Medicine

基　　金：江西省自然科学基金资助项目(0340078)

摘　　要：目的研究肠内免疫营养联合重组人生长激素(rhGH)对烫伤大鼠营养代谢、免疫功能及炎性反应的影响及其机制。方法64只SD大鼠制备30%总体表面积(TBSA)度烫伤模型,然后随机分为肠内免疫营养(EIN)组及EIN联合rhGH(EIN+rhGH)组,两组给予等热量肠内营养液。分别于伤后1、4、7、10d(n=8)抽取静脉血,检测营养代谢、T淋巴细胞亚群、血清免疫球蛋白和有关炎性介质指标。另取8只大鼠检测上述各指标作为伤前对照值。结果与EIN组比较,EIN+rhGH组在伤后各时间点的血清总蛋白、白蛋白、转铁蛋白、CD3+、CD4+、CD4+/CD8+、IgG、IgM和IgA均有所升高,但差异均无显著性(P均>0.05),CD8+有所降低,差异也无显著性(P均>0.05);与EIN组比较,EIN+rhGH组在伤后4、7、10d血清内毒素(LPS)、白细胞介素6(IL6)和肿瘤坏死因子α(TNFα)明显降低(P<0.05或P<0.01)。结论EIN联合rhGH可以改善烧伤后营养代谢和免疫功能,减少炎性反应,其作用要优于单纯肠内免疫营养。Objective To investigate the effects of enteral immunonutrition （EIN） combined with recombinant human growth hormone （rhGH） on nutrition metabolism, immunological function and inflammatory response in rats with severe scald. Methods Sixty -four SD rats subjected to 30% total body surface area （TBSA） It degree scalds were randomly divided into EIN and EIN combined with recombinant human growth hormone （EIN＋rhGH） groups. Animals of both groups were ted isocaloric enteral nutrition. Nutrition metabolism, T lymphocyte subsets, serum levels of immunoglobulins and inflammatory mediators were determined on the 1, 4, 7 and 10 postinjury day （n 8）, Another 8 rats served as controls, and the above mentioned parameters were also determined. Results Total protein （TP）, albumin （Alb）, tiansferrin protein （TFP）, CD3^＋, CD4^＋, CD4^＋/CD8^＋, IgG, IgM and IgA in group EIN ＋ rhGH were slightly higher than those of group EIN （all P2〉0.05） at all time points after the injury, while CD8＋ slightly lowered （P〉0.05）. Serum levels of lipopolysaccharide （LPS） ,interleukin - 6 （IL 6） and tumor necrosis factor - α （TNF -α） plummeted significantly （P〈0.05 or P〈0.01 ）on 4 th, 7 th and 10 th postinjury day compared with group EIN. Conclusion Our findings indicate that EIN combined with rhGH is superior to EIN alone in the early stage of severe scald in rats, not only ameliorate the disturbance in metabolism and immunological function, but also reduce inflammatory response.

关 键 词：肠内免疫营养 重组人生长激素 烫伤 营养代谢 大鼠 CD4^+/CD8^+ 联合治疗 

分 类 号：R644[医药卫生—外科学]