日本血吸虫单克隆抗独特型抗体NP30重链(CDR3)_6基因的构建表达及初步鉴定  

Construction,expression and characterization of H chain (CDR3)_6 gene of anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum

作　　者：焦永军[1] 岳国锋[2] 徐玮[2] 宋晓彤[1] 朱进[1] 管晓虹[1] 冯振卿[2] 

机构地区：[1]南京医科大学医学分子生物学研究所,南京210029 [2]南京医科大学病理学系

出　　处：《中国血吸虫病防治杂志》2006年第1期15-18,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家高技术研究发展计划(863计划)(2002AA214181)

摘　　要：目的设计、构建日本血吸虫单克隆抗独特型抗体NP30重链(H链)CDR3区6倍重复表位基因(CDR3)6,并对表达产物进行初步鉴定。方法克隆NP30H链(CDR3)6基因,导入PET-28(a)载体并在E.coliBL21中表达;表达产物经纯化后用ELISA方法测定活性。结果(CDR3)6基因被成功构建、表达,表达产物经ELISA检测证实其可与抗NP30抗体NP48及日本血吸虫病人血清反应。结论(CDR3)6部分保留了抗独特型抗体表位的亲和性和特异性。Objective To design and construct H chain （CDR3）6 gene of anti-idiotypic monoclonal antibody NP30 of Schistosoma japonicum, and to characterize the immunological activity of its product. Methods The H chain （CDR3）6 gene of anti idiotypic monoclonal antibody NP30 was cloned into expression vector PET-28（a）. The recombinants was transformed into E. coli BL21 to get expressed, purified and the antigen immunological activity of expressed product was detected by ELISA. Results The H chain （CDR3）6 gene has been successfully constructed, expressed, and its product has been found to react with anti-NP30 antibody （NP48） and schistosomasis patient sera by ELISA. Conclusion The H chain （CDR3）6 fragment of anti-idiotypic monoclonal antibody NP30 could partially keep the affinity and specificity of its parent epitope.

关 键 词：日本血吸虫 抗独特型抗体 H链 CDR3 克隆 表达 

分 类 号：R383.24[医药卫生—医学寄生虫学]