埃及伊蚊蚊幼肠道cDNA合成研究  

Studies on cDNA Synthesis of Aedes aegypti Larvae Gut

作　　者：孙建光[1] 高俊莲 Soberon Mario 

机构地区：[1]中国农业科学院农业资源与农业区划研究所农业微生物资源与利用研究室,北京100081 [2]Instituto de Biotecnologia,Universidad Nacional Autonoma de Mexico

出　　处：《中国媒介生物学及控制杂志》2006年第1期34-36,共3页Chinese Journal of Vector Biology and Control

基　　金：国立墨西哥大学博士后基金的支持

摘　　要：目的合成埃及伊蚊蚊幼肠道cDNA,为进一步构建伊蚊蚊幼肠道cDNA基因文库奠定基础。方法饲养埃及伊蚊,收集蚊幼肠道并提取mRNA,合成cDNA。结果成功地合成了埃及伊蚊蚊幼肠道cDNA 6μg,纯度达到A260/A2801.95,片段大于400 bp。结论使用Invitrogen Life technologies公司的FastTrack试剂盒和Copy Kit试剂盒成功地合成了高质量的cDNA,为伊蚊蚊幼肠道cDNA基因文库构建奠定了基础。Objective Synthesize Aedes aegypti larvae gut cDNA for library construction. Methods Feed Ae. aegypti, collect larvae gut, isolate RNA and mRNA, synthesize cDNA. Results 6μg larvae gut cDNA of Ae. aegypti were successfully synthesized, A260/A280 is 1.95, DNA fragment size is larger than 400 bp. Conclusion Ae. aegypti larvae gut cDNA was synthesized in good quality with FastTrack Kit and Copy Kit of Invitrogen Life technologies. This may be a good foundation for Ae. aegypti larvae gut cDNA library construction.

关 键 词：埃及伊蚊 蚊幼 CDNA合成 肠道 

分 类 号：R384.1[医药卫生—医学寄生虫学]