天花粉蛋白对不同小鼠品系T细胞亚群分化的影响  

Differential effects of Trichosanthin on T subset differatiation in two inbred mouse strains

作　　者：焦志军[1] 王保龙[1] 路丽明[1] 丁庆[1] 周晓荣[1] 江阳[1] 周芸[1] 辛利军[1] 周光炎[1] 

机构地区：[1]上海交通大学医学院上海市免疫学研究所,上海200025

出　　处：《现代免疫学》2006年第1期12-16,共5页Current Immunology

基　　金：国家973项目子课题资助项目(2001CB510003);国家自然科学基金重点资助项目(30530690);上海市科委基金资助项目(05DZ19734)

摘　　要：为进一步探讨不同品系小鼠对天花粉蛋白(Tk)免疫抑制敏感性不同的机制,选择Tk高敏感品系(C57BL/6)和低敏感品系(C3H/He)小鼠,建立OVA特异性体外增殖系统,应用流式细胞术胞内细胞因子染色技术分析Th和Tc细胞亚群,real-time PCR检测细胞因子及相关转录因子的mRNA水平。发现Tk作用下,高敏感品系小鼠C57BL/6 IL-4阳性T细胞明显增多,表达IFN-γ阳性T细胞则显著减少。而低敏感品系C3H/He相关变化不明显。T细胞分化相关细胞因子IL-4、IL-10和IFN-γ及转录因子T-bet、gata-3的基因表达水平也呈现类似的格局。提示Tk发挥免疫抑制作用可能与其诱导的以Th2/Tc2为主的T细胞亚群有关,且这种诱导特定细胞亚群的作用在小鼠中和H-2b基因型相关。To investigate the mechanism underlying the different susceptibility in mouse to the Trichosanthin （Tk） induced im mune suppression, OVA-specific proliferation system was established. The T subsets, the mRNA expression of eytokines as well as the cytokine-related transcription factors were detected by flow cytometry and real-time quantitative PCR. Within the OVA-specific T cells, Tk treatment significantly increased the propotion of IL-γ. positive T cells and depressed that of IFN-γ positive T cells only in high susceptible mouse strain（C57BL/6, H-2b）, but not in low susceptible mouse strain（C3H/He, H-2k）. The expression of T cell differentiation-related cytokines（IL-4, IL-10 and IFN-γ） and transcription factors（T bet and gate3） showed similar patterns in two strains. The results suggest that Triehosanthin-indueed suppression for OVA-induced lymhoproliferation is mediated by T subset（Th2/Te2） and associated with the H-2^h background.

关 键 词：天花粉蛋白 细胞因子 转录因于 T细胞亚群 小鼠近交系 

分 类 号：R392.11[医药卫生—免疫学]