IVIG对新生儿脐带血单核吞噬细胞表面分子的影响  

Effects of intravenous immunoglobulin upon the surface molecules on the neonatal umbilical cord blood monocytes/macropahages

作　　者：王美红[1] 陈同辛[1] 朱亚忠[1] 李庆生[1] 

机构地区：[1]上海交通大学附属新华医院上海市儿科医学研究所免疫/肿瘤研究室,上海200092

出　　处：《现代免疫学》2006年第1期56-59,共4页Current Immunology

基　　金：国家自然科学基金项目(39600158);上海市高等学校科学技术发展基金资助项目(03BK11)

摘　　要：为从脐带血单核吞噬细胞(Mo/Mφ)表面CD14、HLA-DR和CD86分子表达的角度来探讨IVIG对新生儿免疫抑制作用,将IVIG、GM-CSF和LPS不同组合对Mo/Mφ进行刺激培养,利用双色免疫荧光抗体标记-流式细胞技术检测Mo/Mφ表面CD14、HLA-DR和CD86分子的表达。加入IVIG处理培养的脐带血Mo/Mφ表面CD14、HLA-DR和CD86分子的阳性细胞比率和相对荧光密度均明显降低,同时CD14+HLA-DR+和CD14+CD86+细胞的比率明显降低。IVIG可通过抑制脐带血Mo/Mφ表面CD14、HLA-DR和CD86分子的表达而抑制其抗原提呈能力,这可能是IVIG免疫抑制的机理之一。In order to investigate the inhibitory mechanism of intravenous immunoglobulin（IVIG） upon the surface molecules （CD14, HLA-DR, CD86） on neonatal umbilical cord blood monocytes/macrophages （Mo/Mφ）, this kind of Mo/Mφ was stimulated with different combinations of IVIG, GM-CSF and LPS, and the expressions of CD14, HI.A DR and CD86 on Mo/Mφ were detected by means of dual color immunofluorescence antibody staining and flow cytometry （FCM）. In addition, the results were compared with those of the adults. It was found that the percentages of CD14 ＋, HLA-DR ＋ and CD86 ＋ cells and the relative intensity of fluorescence were significantly re-duced when the Mo/Mφ were treated with IVIG. Also, IVIG could inhibit the activation of the Mo/Mφ induced by GM CSF and LPS. It is concluded that IVIG can suppress the capacity of antigen presentation of umbilical cord blood Mo/Mφ, and this may be the mechanism for the inhibitory effect of intravenous immunoglobulin.

关 键 词：新生儿 静脉免疫球蛋白 单核吞噬细胞(Mo/Mφ) CD1 4 HLA—DR CD86 

分 类 号：R725[医药卫生—儿科]