三乙酰莽草酸对大鼠局灶性脑缺血再灌注过程中血浆血管活性物质量和脑髓过氧化物酶活性的抑制作用(英文)  

Inhibitory effect of triacetylshikimic acid on plasma contents of vasoactive substances and brain myeloperoxidase activity during focal cerebral ischemia-reperfusion in rats

作　　者：李欣志[1] 种兆忠[2] 徐秋萍[2] 孙建宁[2] 刘建勋[1] 

机构地区：[1]中国中医科学院西苑医院基础医学研究中心,北京100091 [2]北京中医药大学中药学院药理教研室,北京100210

出　　处：《中国药理学与毒理学杂志》2006年第1期13-18,共6页Chinese Journal of Pharmacology and Toxicology

基　　金：国家科技部"新药研究与产业化开发项目"(96-901-01-35);国家重点基础研究发展计划(973项目,G1999054400)~~

摘　　要：目的探索血浆内血管活性物质和白细胞浸润在大鼠局灶性脑缺血再灌损伤中是否影响力不同及三乙酰莽草酸（TSA）的保护作用。方法线栓法制备大鼠大脑中动脉缺血90min再灌注3～48h模型。分别于缺血开始和缺血60min时给予＇FSA50～200mg·kg^-1。分别用荧光分光光度法和放射免疫法测定血浆5-羟色胺（5-HT）和血栓烷素B2（TXB2）含量，化学法测定脑皮层中髓过氧化物酶（MPO）活性。结果大鼠脑缺血再灌注3～24h时血浆5-HT和TXB2含量及脑MPO活性呈时间依赖性升高，48h后5一HT，TXB2含量降至假手术组水平，而MPO活性仍明显高于未缺血侧脑皮层。TSA（100和200mg·kg^-1）可显著抑制缺血90min再灌注24h时血浆5-HT和TXB2含量及脑MPO活性增高。结论大鼠局灶性脑缺血再灌注过程中血浆中血管活性物质量和脑组织中MPO活性表现出不同的时相变化，并且对脑损伤影响力不同，TSA可有效保护缺血脑组织。AIM To explore whether plasma vasoactive substances and neutrophil infiltration make different contribution in cerebral ischemia-reperfusion injury and protective effect of triacetylshikimic acid （ TSA ）. METHODS The rat models of ischemia 90 min and reperfusion 3 -48 h were prepared with middle cerebral artery occlusion. TSA 50- 200 mg· kg^-1 were given （ig） immediately and 60 min again after the onset of ischemia. Serotonin and thromboxane B2 （TXB2 ） concentrations in plasma were detected by fluorescence spectrophotometry and radioimmunoassay respectively. Myeloperoxidase （MPO） activity in brain tissue was quantified by chemical analysis. RESULTS At 3 -24 h after reperfusion, the concentrations of plasmic serotonin, TXBE, and brain MPO activity increased obviously in a time-dependent manner. At 48 h after reperfusion, the concentrations of serotonin and TXB2 decreased to the same level of sham. Nevertheless, brain MPO activity remained more elevated than the contralateral cortex. At 24 h after reperfusion, TSA （100 and 200 mg·kg^-1） was shown to possess the ability to inhibit the increased ptasmic serotonin, TXB2 concentrations, and brain MPO activity induced by focal cerebral ischemia-reperfusion. CONCLUSION Vasoactive substances in plasma and MPO activity in brain tissue show different time courses during focal cerebral ischemia-reperfusion and make different contribution to brain damage. TSA is effective to protect the ischemic brain tissue from ischemiareperfusion injury.

关 键 词：三乙酰莽草酸 脑缺血 5-羟色胺 血栓烷素B2 过氧化物酶 

分 类 号：R96[医药卫生—药理学]