落新妇甙对心脏移植排斥反应中细胞因子表达的影响  

Effects of astilbin on the expression of IL-2,γ-IFN,IL-4,IL-10,IL-12 and IL-18 in mice heart transplantation rejection

作　　者：高思海[1] 李平[1] 陈涛[1] 潘铁成[1] 赵金平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院胸心外科,武汉430030

出　　处：《中华小儿外科杂志》2006年第2期87-89,共3页Chinese Journal of Pediatric Surgery

基　　金：国家自然科学基金资助项目(30300323)

摘　　要：目的探讨落新妇甙对心脏移植排斥反应中细胞因子表达的影响。方法采用小鼠颈部心脏移植模型,随机分为3组:对照组,移植组,落新妇甙组。于术后第5d取移植心脏,用逆转录聚合酶链式反应(RT-PCR)法观察IL-2、γ-IFN、IL-4、IL-10、IL-12、IL-18的表达情况。结果移植组IL-2、γ-IFN、IL-12、IL-18表达明显升高,IL-4、IL-10表达显著降低(与对照组相比,P<0.01)。落新妇甙组IL-2、γ-IFN、IL-12、IL-18表达明显降低,而IL-4、IL-10表达显著升高(与移植组相比,P<0.01)。结论落新妇甙抑制心脏移植排斥反应主要与其抑制IL-2、γ-IFN、IL-12、IL-18等Th1型细胞因子的表达,促进Th2型细胞因子IL-4、IL-10的表达,使免疫反应由Th1型向Th2型偏移有关。Objective To investigate the effects of Astilbin on the expression of IL-2,γ- IFN, IL-4, IL-10, IL-12 and IL-18 in mice heart transplantation rejection. Methods Model of mice cervical heterotopic heart transplantation was established. The mRNA of IL-2, γ-IFN, IL-4, IL-10, IL- 12 and IL-18 were measured by RT-PCR at 5 days after transplantation in control, transplant and Astilbin group. Results Compared to the control group, the mRNA levels of IL-2, γ- IFN, IL-12 and IL-18 were significantly increased while those of IL-4 and IL-10 were significantly decreased in transplant group （P〈0. 01）. In Astilbin group, the mRNA levels of IL-2, γ-IFN, IL-12 and IL-18 were signifi cantly decreased while IL-4 and IL-10 were significantly increased （P〈0. 01） compared to the transplant group. Conclusions Astilbin can decrease the expression of IL-2, γ- IFN,IL- 12 and IL-18 while increase the expression of IL-4 and IL-10 in mice heart transplantation rejection.

关 键 词：心脏移植 移植物排斥 细胞因子 

分 类 号：R26[医药卫生—中医外科学]