抗人DR5单抗与阿霉素联合对人肝癌细胞SMMC-7721协同杀伤效应  

Synergistic effect of anti-human DR5 monoclonal antibody and adriamycin on SMMC-7721 cells

作　　者：张军[1] 马远方[1] 刘广超[1] 李淑莲[1] 卢锋[1] 

机构地区：[1]河南大学细胞与分子免疫学重点实验室,开封475001

出　　处：《中国免疫学杂志》2006年第2期133-136,共4页Chinese Journal of Immunology

基　　金：河南省杰出人才创新基金项目(0321001800);河南省医学科技创新人才工程项目(2002119)

摘　　要：目的:探讨mDRA-6对肝癌细胞系SMMC-7721致凋亡作用,及阿霉素与mDRA-6联合协同杀伤效应与机制。方法:常规培养肝癌细胞SMMC-7721,流式细胞术检测细胞表面DR5的表达率。Hoechst 33258染色观察SMMC-7721细胞形态变化;MTT法检测细胞毒性作用;流式细胞术定量分析凋亡细胞率。结果:(1)mDRA-6能够诱导SMMC-7721细胞凋亡,在1.89μg/m l浓度下可杀伤36%的细胞,增加mDRA-6浓度,凋亡作用无明显增加;(2)SMMC-7721细胞对阿霉素敏感,存在浓度依赖性;(3)阿霉素与mDRA-6联合对SMMC-7721细胞具有协同杀伤作用,2μg/m l的mDRA-6与40 ng/m l的阿霉素联合杀伤60%SMMC-7721,Hoechst 33258染色和Annexin V/PI染色证实杀伤作用是通过细胞凋亡实现的。结论:mDRA-6能够诱导肝癌细胞SMMC-7721凋亡,阿霉素与mDRA-6联合具有协同作用。Objective：To determine the sensitivity of SMMC-7721 ceils to mDRA-6, the synergistic damage effect of mDRA-6 and adriamycin on human hepatoceilular carcimoma ceil lines and its possible mechanism. Methods：SMMC-7721 ceils were cultured with RPMI1640 medium in regular condition. The morphology was observed by microscope. Cytotoxicity was examined by MTT assay. Apoptosis were detected by flow cytometry. Results： （ 1 ）The apoptosis of SMMC-7721 ceils could be induced by mDRA-6, 1.89 μg/ ml of mDRA-6 cound kill 35% of the ceils ： （2） Concentration-dependent cytotoxicity of adriamycin was exhibited in SMMC-7721 ceils ; （3）The combination of mDRA-6 and adriamycin exhibited synergistic effect on SMMC-7721 cells. 2 μg/ml of mDRA-6 and 40 ng/ml of adriamycin killed 50% SMMC-7721. Conclusion：MDRA-6 can induce SMMC-7721 ceil apoptosis. The combination of mDRA-6 and adriamycin exhibit synergistic effect on SMMC-7721 ceils.

关 键 词：抗人DR5单克隆抗体(mDRA-6) 阿霉素 协同杀伤作用 细胞凋亡 

分 类 号：R392.5[医药卫生—免疫学]