不同缺血预处理对未成熟心肌细胞功能的影响  

Experimental study of immature myocardial cell function with different ischemic preconditioning in neonatal rabbits

作　　者：孙忠东[1] 宋玉娥[1] 欧焕峰 夏家红[3] 刘成硅[3] 

机构地区：[1]青岛大学医学院附属青岛市立医院,山东青岛266011 [2]中国重汽集团公司医院,山东济南250031 [3]华中科技大学同济医学院附属协和医院心血管外科,湖北武汉430022

出　　处：《中国心血管杂志》2006年第1期3-4,38,共3页Chinese Journal of Cardiovascular Medicine

摘　　要：目的比较不同方法的缺血预处理对未成熟心肌细胞功能的影响。方法采用Langendorff离体心脏灌注模型。分为4组:缺血/再灌注(I/R)组,离体心脏灌注15min转为工作心15min后停灌45min,恢复灌注15min改为工作心30min;心脏缺血预处理(MIP)组:离体心脏灌注15min转为工作心15min后反复2次缺血5min/再灌注5min,然后重复I/R组缺血/再灌注方法;肾缺血预处理(RIP)组:反复三次阻断左肾动脉5min,放开5min,切取心脏,重复I/R组方法;双下肢缺血预处理(DLIP)组:反复3次捆扎双下肢5min,松开5min,切取心脏,重复I/R组方法。以血清肌酸激酶(CK)和乳酸脱氢酶(LDH)漏出率、心肌组织三磷腺苷(ATP)和丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、心肌细胞内Ca2+含量、心肌线粒体Ca2+-ATPase活性及其Ca2+含量、心肌线粒体合成三磷腺苷能力[ATP]m作为观察指标。结果MIP、RIP及DLIP组ATP含量、SOD活性、心肌线粒体Ca2+-ATPase活性、[ATP]m均高于I/R组(P<0.01),MDA含量、CK、LDH漏出率、心肌细胞内Ca2+含量、心肌线粒体Ca2+含量均低于I/R组(P<0.01)。结论肾缺血预处理、双下肢缺血预处理与心脏缺血预处理有同等的心肌细胞保护作用。Objective To determine the immature myocardial cell protection effects with different isehemic preconditioning. Methods Isolated perfused neonatal rabbit hearts form group ischemic/reperfusion （I/R）, group myocardial isehemic preconditioning （MIP）, group renal ischemic preconditioning（RIP） and group double limbs ischemic preconditioning （DLIP） were underwent 45 min ischemia followed 45 min reperfusion before group I/R no IP, group IP with 2 x IP（5 min ischemia followed 5 min reperfusion ）, group RIP with 3 × IPC （fightkidney artery was clamped 5 rain followed 5 min reperfusion）, group DLIP with 3 × IPC（double limbs were obstructed 5 rain followed 5 rain reperfusion）, but group normal controll（NC） no ischemia only perfused KH solutions 70min. MWC, LDH and CK leakage, MDA and ATP content, SOD activity, myocardial cell Ca^2 ＋ （ [ Ca^2 ＋ ] c ） content, mitothondial Ca^2 ＋ content （ [ Ca^2＋ ] m ）, Ca^2＋ -ATPaseactivity of mitothondia , synthesizing ATP activity of mitothondia（ [ ATP] m） were tested. Results The ATP content, SOD activity, synthesizing ATP activity of mitothondia （ [ATP]m）, mitothondial Ca^2＋ content （ [Ca^2＋ ]m） and Ca^2＋-ATPase activity of mitothondia（ [Ca^2＋ -ATPase]m） in IP, DLIP and RIP group were higher than that of I/R group. MWC,MDA content , LDH and CK leakage, myocardial cell Ca^2＋ （ [ Ca^2＋ ] c} content and mitothondial Ca^2＋ cofitent （ [ Ca^2 ＋ ] m） in IP, DLIP and RIP group were lower than that of I/R group. Conclusion RIP and DLIP had the same cardioprotection to immature myocardial Cell as IP.

关 键 词：缺血预处理 未成熟心肌 心肌保护 

分 类 号：R654.2[医药卫生—外科学]