幽门螺杆菌的培养与保存  

Study on Cultivation and Preservation of Helicobacter Pylori

作　　者：谢献胜[1] 王锋[2] 陆桂平[1] 卢炜[1] 崔山[2] 周冬仁[2] 

机构地区：[1]江苏畜牧兽医职业技术学院,江苏泰州225300 [2]南京农业大学动物科技学院,江苏南京210095

出　　处：《泰州职业技术学院学报》2006年第1期51-53,共3页Journal of Taizhou Polytechnic College

基　　金：江苏高校高新技术产业发展指导性计划项目(JHZD03-028)

摘　　要：采用不同培养基对幽门螺杆菌(Helicobacter pyloric,Hp)进行培养,研究了不同培养基对Hp生长的影响;并评价了Hp低温保存与复苏效果。结果表明:(1)采用固体培养Hp时,在微需氧条件下,培养基分别为哥伦比亚琼脂+混合抗生素+6%脱纤维绵羊血,哥伦比亚琼脂+混合抗生素+6%马血清,37℃培养3d,均可培养出Hp;在严格无菌的条件下,不添加混合抗生素,培养基为哥伦比亚琼脂+6%马血清,对细菌的生长没有影响,且不会造成杂菌污染(。2)采用液体培养Hp时,培养基为布氏肉汤基础加混合抗生素和6%马血清,Hp增菌迅速。菌株采取冷冻保存液法保存于-70℃￣-80℃低温条件下,可以成功复苏。Different cultivation media were tested to cultivate and preserve helicobacter pylori （Hp）. Results：l.Solid cultivation medium for Hp： Colombian agar medium with mixed antibiotic added with 6% sheep blood and 6% horse serum, respectively if under 37℃ microaeropgilic condition, after 3 days, could cultivate Hp; If under asepsis condition, without mixed antibiotic, germ growth was not influenced and bacteria pollution was not caused. 2.Liquid cultivation media for Hp： Bromfield medium added with mixed antibiotic and 6% horse serum could promote proliferation of Hp. Conclusion： If Hp was preserved under -70℃ to - 80℃, it could be successfully anabiotic.

关 键 词：幽门螺旋杆菌 培养 保存 

分 类 号：R377[医药卫生—病原生物学]