脱细胞猪肺动脉带瓣管道的支架材料研究  

A study on porcine acellular cardiac scaffold

作　　者：闫鹏[1] 胡盛寿[1] 任兵[1] 周建业[1] 高峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院瓣膜研究室,北京100037

出　　处：《中华胸心血管外科杂志》2005年第5期286-288,共3页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金资助(30370390)

摘　　要：目的研究心血管组织在经过适当脱细胞处理后的组织结构和生物学特性。方法对带瓣猪肺动脉管道进行脱细胞处理,所得组织和作为对照的新鲜天然组织分别进行HE染色、ETVG染色、免疫组化染色及扫描电镜检查,并测定各种大分子物质的含量;在小鼠皮下移植6周后测定组织钙含量及钙定位染色。结果脱细胞后的组织内未见细胞结构,基质结构基本保持。胶原和弹性蛋白含量增加。脱细胞后的组织在小鼠皮下移植后钙含量较天然组织明显下降。结论经过合适的脱细胞处理后,可以去除几乎所有的细胞成分,基本保留的基质结构,能显著减轻在异种移植时引起的钙化。Objective To study the change of the structural and biological properties of the cardiovascular tissue after cell extracting. Methods The native and treated pulmonary cusp and pulmonary artery wall were evaluated by HE stain, ETVG stain, immtmochemistry stain and scan electron microscopy. The contents of the main macro-molecules were measured. The content and position of calcium was investigated by subcutaneous implantation in a rat model. Results The acclimatization procedure resulted in an almost complete removal of the original cells while the construction of the matrix remained. The contents of colleagen and elastin increased while soluble GAG reduced. The content of calcium in acellular tissue by xenogenic implantation was less obviously than that in fresh one. Conclusion A suitable acellularization procedure can remove all cells and most soluble proteins, and matrix remains nearly intact, so as to alleviate obviously calcification via xenogenic implantation in vivo.

关 键 词：肺动脉 生物假体 细胞去除术 动物实验替代试验 钙质沉着症 脱细胞处理 带瓣管道 材料研究 猪肺 组织结构 

分 类 号：R318.08[医药卫生—生物医学工程]