人类羊膜细胞表达神经干细胞特异性蛋白研究  

Specific proteins of neural stem cell expressed by human amnion cells

作　　者：蔡哲[1] 潘琳[1] 舒峻[1] 张岚[1] 郭艳茹 耿同超[2] 牟亮[3] 左萍萍[3] 

机构地区：[1]中日友好医院临床医学研究所免疫学研究室,北京市100029 [2]清华大学附属玉泉医院神经内科,北京市100049 [3]中国医学科学院基础医学研究所药理学研究室,北京市100005

出　　处：《中国康复理论与实践》2005年第12期965-967,i0001,共4页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市自然科学基金重点项目(No.5041002);国家自然科学基金项目(No.30471773)。

摘　　要：目的利用神经干细胞特异性标志蛋白鉴定人类羊膜组织中多分化潜能神经前体细胞的存在。方法利用免疫组织化学法检测人类羊膜组织和培养人类羊膜细胞中巢蛋白(nestin)、胶质原纤维酸性蛋白(GFAP)、musashi-1、波形蛋白(vi mentin)和PSA-NCAM等神经干细胞特异性标志蛋白的表达。结果人羊膜组织中存在nestin/GFAP双阳性细胞,此外,还表达musashi-1、vi mentin和PSA-NCAM等神经干细胞特异性标志蛋白;培养羊膜细胞中存在vi mentin和PSA-NCAM阳性细胞,以及nestin/GFAP双阳性细胞。结论羊膜组织和培养羊膜细胞中有神经干细胞特异性标记蛋白的表达。Objective To characterize the neural progenitor cell in the human amnion mesenchyme and epithelial layer with specific mark proteins of neural stem cell. Methods Expressions of specific mark proteins of neural stem cell including nestin, glial fibrillary acidic protein （GFAP）, musashi-1, vimentin and PSA-NCAM in human amnion tissue and cultured amniotic ceils were determined by immunohistochemistry and immunofluorescence staining. Results Expressions of pluripotent neural cell specific makers （nestin, musashi-1, vimentin and PSA-NCAM） were detected in the human amnion mesenchyme and epithelial layer. In addition, cultured amniotic cells were expressed several neural stem cell specific markers including nestin, GFAP and PSA-NCAM. Nestin＋ and GFAP＋ double positive ceils were identified in the human amnion tissue and cultured amniotic ceils by immunohistochemistry and immunofluorescence staining. Conclusion Specific mark proteins of neural stem cell are expressed in human amnion tissue and cultured amniotic ceils.

关 键 词：人类羊膜细胞 神经前体细胞 巢蛋白 MUSASHI-1 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]