乙酰二脱水卫矛醇对L1210细胞Caspase-3活性的影响  

Effecct of diacetyldianhydrogalactitol on the activity of Caspase-3 in L1210 cells

作　　者：邓晓慧[1] 范文燕[2] 郑风劲[1] 杨锦南[2] 刘小康[1] 

机构地区：[1]四川大学基础医学与法医学院药理教研室,成都610041 [2]新乡医学院形态学教研室,新乡453003

出　　处：《四川生理科学杂志》2005年第4期159-161,共3页Sichuan Journal of Physiological Sciences

摘　　要：目的:探讨半胱氨酶-3(Caspase-3)在二乙酰二脱水卫矛醇(d iacetyld ianhydrogalactitol,DADAG)诱导小鼠白血病L1210凋亡过程中的作用。方法:MTT法观察DADAG对L1210细胞的生长抑制作用;透射电镜检测细胞凋亡;Caspase-3试剂盒测定细胞内Caspase-3的酶活性。结果:DADAG对L1210细胞有较强的体外增殖抑制作用,其IC50值为24.6 mg/L;DADAG可诱导L1210细胞发生凋亡及时间依赖性地升高Caspase-3酶活性。结论:Caspase-3在DADAG诱导的细胞凋亡中可能起重要作用。Objective： To investigate the role of Caspase-3 in DADAG-indueed apoptosis of mouse L1210 leukemia. Methods： The growth inhibiton was analysed by MTT assay. DADAG-induced apoptosis in L1210 ceils was identified by electron microscopy; Caspase-3 activity was measured by ApoAlert CPP32 colorimetrie assay kit. Results： DADAG showed potent antiproliferation on L1210 cells with the IC50 of 24.6 mg/L in vitro. Meanwhile apoptosis was induced in targeted cells. DADAG time-dependently increased the activity of Caspase-3. Conclusion： Caspase-3 might play an important role in DADAG-induced apoptosis of L1210 ceils.

关 键 词：二乙酰二脱水卫矛醇 L1210细胞 凋亡 半胱氨酶-3 CASPASE-3活性 Caspase-3酶活性 白血病L1210 细胞凋亡 生长抑制作用 增殖抑制作用 

分 类 号：R96[医药卫生—药理学]