斑马鱼胚胎发育过程中Mef2c的表达  

Mef2c gene expression profile during zebrafish embryogenesis

作　　者：刘学飞[1] 王跃祥[1] 蒋璆[1] 钱林溪[1] 高广文[1] 董永新[1] 钟涛 宋后燕[1] 

机构地区：[1]复旦大学上海医学院生物化学与分子生物学系-分子医学教育部重点实验室,上海200032 [2]美国Vanderbilt大学医学院心血管研究室,tn37027

出　　处：《复旦学报（医学版）》2006年第1期89-91,F0002,共4页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金海外青年学者合作研究基金(30328009);复旦大学特聘教授启动基金资助

摘　　要：目的探讨Mef2c在斑马鱼胚胎骨骼肌和心肌中表达的时相变化。方法收集不同发育时期的AB野生型斑马鱼胚胎,采用整体原位杂交技术检测Mef2c在骨骼肌和心肌的转录情况。结果Mef2c在体节中的表达约在13hpf,而在心脏中的表达出现在13hpf以后,与体节中的表达时间基本平行。另外,Mef2c在体节和心脏中的表达一直维持到48hpf。结论明确了Mef2c在斑马鱼胚胎骨骼肌和心肌发育的表达时相变化,为研究斑马鱼早期骨骼肌和心肌发育的调控机制提供了一定的理论基础。Purpose To analyze the expression pattern of Mef2c during zebrafish embryogenesis. Methods We collected all the phases of zebrafish embryos to detect the expression pattern of Mef2c in the somite and the heart during zebrafish embryogenesis by whole mount in situ hybridization. Results We first detected Mef2c expression at 13 hours post-fertilization in the somite that is the latest expression in the somite of Mef2c family. Expression of Mef2c in heart was detected as early as in somite and continued for 48 hours post-fertilization. Conclusions We made clear the expression pattern of Mef2c during zebrafish embryogenesis in order to make sure that Mef2c plays important roles in the regulation of cardiac and skeletal muscle development.

关 键 词：Mef2c 斑马鱼 反义RNA探针 整体原位杂交 

分 类 号：Q959.483[生物学—动物学]