唐菖蒲球茎芽高频再生体系的建立  

ESTABLISHMENT OF A HIGH FREQUENCY REGENERATION SYSTEM OF GLADIOLUS CORM BUDS

作　　者：艾丽皎[1] 李名扬[2] 

机构地区：[1]重庆市园林绿化科学研究所,重庆400038 [2]西南大学花卉研究所,重庆400716

出　　处：《西南农业大学学报（自然科学版）》2005年第6期844-846,共3页Journal of Southwest Agricultural University

摘　　要：将带芽的唐菖蒲子球茎切块接种在附加2,4-D 3.0 mg/L的MS基本培养基上诱导愈伤组织的发生。愈伤组织转至MS+6-BA 0.5 mg/L+NAA 0.1 mg/L诱导芽的分化。丛生芽继代增殖在MS+6-BA 0.5 mg/L+NAA 0.1mg/L上,增殖系数最高。生根培养以1/2 MS+NAA 0.1 mg/L效果最佳。Slices of dormant cormlets with buds of Gladiolus hybridus were used as explants and inoculated on MS basal medium supplemented with 2,4-D 3.0 mg/L for callus induction. The resulting calli were transferred to media of shoot differentiation and proliferation containing different combinations of 6-BA and NAA. The optimum media for shoot differentiation, proliferation and rooting were MS ＋ 6- BA0.5 mg/L ＋ NAA0.1 mg/L, MS ＋ 6-BA0.5 mg/L ＋ NAA0.1 mg/L and 1/2 MS ＋ NAA0.1 mg/L

关 键 词：唐菖蒲 球茎 芽增殖 再生 组织培养 

分 类 号：S564.3[农业科学—作物学]