Determination of platelet-activating factor by reverse phase high-performance liquid chromatography and its application in viral hepatitis  

作　　者：Hong-Cui Cao, Xiao-Ming Chen, Wei Xu, Department of Infectious Diseases, First Affiliated Hospital, Medical School of Zhejiang University Key Laboratory of Infectious Diseases of Ministry of Public Health, Hangzhou 310003, Zhejiang Province, China 

出　　处：《World Journal of Gastroenterology》2005年第46期7364-7367,共4页世界胃肠病学杂志（英文版）

基　　金：Supported by a Health Foundation of Zhejiang Province, No.2004C083, 2004B068

摘　　要：AIM： To detect the plateletoactivating factor （PAF） and the plasma or serum levels of tumor necrosis factoroa （TNF-α） malondialdehyde （MDA）, endotoxin （ET） and to discuss their significance in various types of viral hepatitis. METHODS： PAF, TNF-α, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography （rHPLC）, bio-assay, ELISA, thiobarbituric acid （TBA）, and limulus lysate test （LLT）, respectively. RESULTS： The rHPLC was more sensitive and specific than bio-assay （r = 0.912, P〈0.01）. The plasma levels of PAF, TNF-α, MDA, and ET in patients with viral hepatitis were higher than those in controls （P〈0.01）. CONCLUSION： rHPLC is more reliable and accurate for the detection of PAF.AIM: To detect the platelet-activating factor (PAF) and the plasma or serum levels of tumor necrosis factor-α (TNF-α) malondialdehyde (MDA), endotoxin (ET) and to discuss their significance in various types of viral hepatitis.METHODS: PAF, TNF-α, MDA, and ET levels in 60 controls, 16 cases of acute viral hepatitis, 71 cases of chronic viral hepatitis, 19 cases of severe viral hepatitis were detected by reverse phase high-performance liquid chromatography (rHPLC), bio-assay, ELISA, thiobarbituric acid (TBA), and limulus lysate test (LLT), respectively.RESULTS: The rHPLC was more sensitive and specific than bio-assay (r = 0.912, P<0.01). The plasma levels of PAF, TNF-α, MDA, and ET in patients with viral hepatitis were higher than those in controls (P<0.01).CONCLUSION: rHPLC is more reliable and accurate for the detection of PAF.

关 键 词：Platelet-activating factor MALONDIALDEHYDE ENDOTOXIN rHPLC Viral hepatitis 

分 类 号：R373.21[医药卫生—病原生物学]