益生菌预防婴幼儿抗生素相关性腹泻及对肠道菌群的影响  

Probiotics in prevention of antibiotic associated diarrhoea and intestinal microflora analysis in children.

作　　者：王小卉[1] 杨毅[1] 曹凌峰[1] 邵彩虹[1] 

机构地区：[1]复旦大学附属儿科医院内科,上海200032

出　　处：《中国儿童保健杂志》2006年第1期21-23,共3页Chinese Journal of Child Health Care

摘　　要：【目的】探讨益生菌对婴幼儿期感染性疾病患儿抗生素应用中腹泻的预防作用及肠道菌群的变化。【方法】89例应用抗生素治疗的下呼吸道感染患儿随机分成两组,一组(益生菌组)同时服用双歧杆菌三联活菌,另一组不用任何微生态制剂为对照组,比较两组腹泻的发生率。分别在抗生素给药前及给药后第3、6 d采集粪便,用荧光定量PCR的方法检测肠道菌群的变化。【结果】44例益生菌组患腹泻5例(发生率为11.3%),45例对照组患儿患腹泻21例(发生率为46.6%)。益生菌组患儿腹泻平均时间(1.30±0.24)d,对照组患儿腹泻平均病程(2.10±0.33)d。抗生素治疗过程中,肠道菌群出现明显改变,益生菌组抗生素治疗后双歧杆菌、乳酸杆菌及大肠杆菌数量较稳定,明显高于对照组(P<0.01)。【结论】益生菌能通过稳定肠道菌群而预防婴幼儿抗生素相关性腹泻。[Objectives] To investigate the difference and signieanee of the intestinal microflora analysis with the use of antibiotics in children. And evaluate efficacy of probiotics in prevention of diarrhoea associated with the use of antibiotics in children. [Methods] Eighty-nine children between 1 months and 2 years of age were enrolled. 44 completed bifid, triple viable capsules was adminstered in randomized trial to children receiving antibiotic therapy in hospital. Real-Time PCR procedures based on 16S rRNA gene sequences specific for Bifidobacterium, lactobacillus and escherichia coli that predominate in the human intestinal tract were developed and used for quantitative detection of these species in children feces results. [Results] The incidence of diarrhea was 11.3% in the probiotics group and 46.6% in the place group of antimierobial therapy （P〈0.05）. The place group were less often determined with bifidobaeteria （P〈0.01）,as compared with the probioties group. Similarly, in the probiotics group the proportions of lactobaeilli were higher than in the place group. In contrast, the place group harbored higher counts Of escherichia coil （P〈0. 01）. [Conclusion] Probiotics can be used to prevent antibiotic associated diarrhoea in children.

关 键 词：益生菌 抗生素相关性腹泻 肠道菌群 

分 类 号：R722[医药卫生—儿科]