转LEA基因烟草的耐盐性分析  

Analysis of Salt Tolerance in Transgenic Tobacco with LEA Gene

作　　者：姜静[1] 于影[1] 赵鑫[1] 刘焕臻[1] 王玉成[1] 杨传平[1] 刘桂丰[1] 

机构地区：[1]东北林业大学林木遗传育种省重点实验室,黑龙江哈尔滨150040

出　　处：《生物技术》2006年第1期16-20,共5页Biotechnology

基　　金：国家转基因植物研究与产业化专项(JY03A1802);国家重点基础研究发展规划(973)项目(G1999016000)

摘　　要：目的:验证柽柳LEA基因的功能,为通过基因工程手段培育耐盐植物提供基础资料。方法:对转LEA基因烟草当代(T0)和子一代(T1)分别进行不同浓度的NaCl胁迫处理,研究转基因烟草的耐盐性。结果:转基因烟草T0代组培苗耐受NaCl的临界浓度为230mmol/L,而对照耐受NaCl的临界浓度为130mmol/L以下;T1代幼苗耐受NaCl的临界浓度为150mmol/L,对照耐受NaCl的临界浓度为100mmol/L以下;在临界浓度转基因烟草的T0代、T1代根系发育良好,生长量明显高于非转基因对照烟草。结论:柽柳LEA基因的转化提高了烟草的耐盐性。Objective：To analysis their fimctions of LEA gene in order to cultivated salt tolerance plants through the genetic engineering means. Methods： To study the salt tolerance of transgenic plants （T0） and their filial generation （T1）, the transgenic tobaccos with LEA gene were treated with different concentration of NaCl. Results： The result of salt tolerane analysis showed that on culture medium, the transgenic tobaccos To can withstand NaCl stress at the critical concentration of 230 mmool/L, while the control group can only withstand of 130 mmol/L. However, the transgenic tobaccos T, can withstand NaC1 stress at the critical concentration of 150mmol/L, while the control group can only withstand of 100mmol/L. Under stress at the critical concentration of NaC1, the transgenic tobacco rooted and growed faster than the control tobaccos did. Conclusion： All the results proved that the LEA gene from Tamarix. sp could enhance the salt tolerance of transgenic tobaccos.

关 键 词：LEA基因 烟草 耐盐性 

分 类 号：Q945.3[生物学—植物学] Q785