油菜试管苗玻璃化发生机理的研究初探  

Study on the Vitrification Mechanism of Rape Hypocotyl in vitro

作　　者：王艳[1] 曾幼玲[1] 贺宾[2] 李金耀[1] 高燕[2] 张富春[1] 

机构地区：[1]新疆生物资源基因工程重点实验室新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]新疆农业科学院经济作物研究所,新疆乌鲁木齐830000

出　　处：《生物技术》2006年第1期75-77,共3页Biotechnology

基　　金：国家"863"项目(No.2004AA227110-2);国家科技攻关项目(No.2001BA901A32)资助

摘　　要：研究了影响甘蓝型油菜离体下胚轴再生过程中试管苗玻璃化产生的几个因子,试验发现:调整激素6-BA与NAA的浓度、增加蔗糖的浓度提高渗透势对玻璃化无影响;降低培养基中NH4+浓度不仅没有有效地防止玻璃苗的发生反而降低了芽的诱导率;采用牛皮纸代替聚乙烯塑料膜作为封口材料的措施受到了地区的限制,没有得到有效的统计数据;选择1%的琼脂浓度可使玻璃化率显著降低55%,但诱芽率在一定程度上降低了6.9%。Some factors ＇affecting vitrification of rape in vitro were studied. The results showed there were no effects by adjusting concentration of 6 - BA and NAA and improving sucrose concentration to increase osmosis. The reduction in NH4 NO3 dose couldn＇ t solve the vitrification effectively and lowered the rate of inducing shoots. Since the method that polyethylene fil as the sealed material of conical flask was substituted for kraft paper was restricted by region climate, valid date couldn＇t be attained. 1% concentration agar could reduce vitrification by 55%, however, at fire same time reducing the inducing shoot by 6.9%.

关 键 词：油菜下胚轴 玻璃化 影响因素 诱芽 

分 类 号：Q943.1[生物学—植物学]