鹅膏毒肽致肝脏损伤的生化指标探讨  

Research on the biochemical index for liver damage induced by amatoxin

作　　者：李玉白[1] 全建设[1] 刘劭钢[2] 

机构地区：[1]湖南环境生物学院医学部,湖南省衡阳市421001 [2]中南大学测试中心,长沙市410083

出　　处：《实用医学杂志》2006年第3期252-253,共2页The Journal of Practical Medicine

基　　金：湖南省卫生厅科研基金资助项目(B2005120);湖南省教委科研基金及学院院长科研基金资助项目(Z05-11);湖南省教育厅科研基金资助(05D030)

摘　　要：目的:探讨敏感的鹅膏毒肽致肝脏损伤的早期临床诊断生化指标。方法:选SD大鼠24只,随机分为4个组(对照组和鹅膏毒肽低、中、高剂量组)。根据动物试验的LD500.3～0.6m g/kg染毒组剂量依次为0.1、0.3、0.5m g/kg,染毒后测定血清羟脯氨酸、氨基己糖含量。结果:中、高剂量染毒组大鼠血清羟脯氨酸、氨基己糖含量显著低于对照组。结论:血清羟脯氨酸、氨基己糖含量可以反映鹅膏毒肽对肝细胞的损伤情况,其可能是鹅膏毒肽中毒早期临床诊断的敏感生化指标。Objective To explore the biochemical indexes for hepatic cell damage induced by amatoxin in the early clinical diagnosis. Methods 24 rats were randomly divided into four groups： control group, low-dose with 0. 1 mg/kg group, median-dose with 0. 3 mg/kg group, high-dose with 0.5 mg/kg group （LD50 0.3-0.6 mg/kg） . The levels of hydroxide proline and hexosamine were detected after administration of amatoxin. Result The levels of hydroxide proline and hexosmaine in administration of amatoxin groups were much lower than those in the control group. Conclusions The serum levels of hydroxide proline and hexosamine can signal hepatic cell damage induced by amatoxin. They may be the sensitive biochemical indexes in the early clinical diagnosis of amatoxin.

关 键 词：鹅膏属 羟脯氨酸 氨基己糖 鹅膏毒肽 生化指标 肝脏损伤 早期临床诊断 大鼠血清 高剂量 染毒后 

分 类 号：R446.11[医药卫生—诊断学]