过敏性紫癜急性期的免疫状态  

The immune state in children with Henoch-Schonlein purpura

作　　者：李文斌[1] 陈凤琴[1] 陈莉[2] 刘泽英[1] 王英荣[1] 薄慕琴[1] 尹娜[1] 

机构地区：[1]河北省人民医院儿童急救中心,石家庄市050071 [2]河北省血液中心

出　　处：《河北医药》2006年第1期13-14,共2页Hebei Medical Journal

摘　　要：目的探讨Henoch-Schonlein purpum,HSP的免疫学发病机制。方法采用直接免疫荧光流式细胞分析技术,多克隆抗体单向免疫扩散法检测SHP患儿急性期外周血单个核细胞CD4+、CD30-／ CD4+、CD30+、Th细胞亚群,血浆免疫球蛋白IgA、IgG、IgM浓度,采用SAS8．0统计软件进行分析。结果 (1)外周血单个核细胞Th1和Th2的含量:HSP组分别为(80．44±1．42)％和(19．56±1．42)％,对照组健康儿童为(89．73±0．52)％和(10．26±0．05)％,t检验P<0．05,显示与健康儿童相比HSP组外周血单个核细胞Th2含量明显较高而Th1含量明显降低。(2)血浆免疫球蛋白的浓度:HSP组IgA、IgG、IgM分别为 (1．616±0．060)g/L、(1．551±1．157)g/L、(1．307±0．144)g/L,对照组健康儿童为(1．077±0．101)g/L、(10． 597±1．283)g/L、(1．272±0．145)g/L,两组间IgA浓度t检验P<0．05,两组间IgG、IgM浓度T检验P>0． 05,显示HSP患儿血浆IgA浓度显著高于正常对照组,血浆IgG、IgM与正常对照组差异无显著性。结论 HSP患儿急性期体内存在T细胞功能紊乱,Th1和Th2的失衡,Th2细胞优势活化。血浆免疫球蛋白 IGA合成异常是产生免疫复合物血管炎的重要原因。Objective To investigate the immunology outbreak mechanism of Henoch-Schordein purpura （HSP）. Methods 4 ml venous blood from the children with HSP and healthy subjects was collected, and the CD4 , CD30^-, Th/CD4^＋ , CD30^＋, Th cell subgroup and the plasma immunoglobulin IgA, IgG, IgM were detected by using the direct immunity fluorescence flow cytometry and polyclonal antibody immunodiffusion method respectively. All the data were analyzed with SAS 8.0 statistical software. Results （ 1 ） Thl and Th2 content in patients with HSP was （80.44±1.42） % and （ 19.56±1.42） % respectively, which was （89.73±0.52） % and （ 10.26±0.05） % in healthy subjects. There was a significant difference between the two groups （ P 〈 0.05）. The content of Th2 in patient with HSP was much higher,however the content of Thl was much lower than those in healthy subjects. （2） The plasma immanoglobulin： the concentration of IgA,IgG, IgM in patients with lISP was （1.616± 0.060） g/L, （1.551±1. 157） g/ L, （ 1. 307±0. 144） g/L respectively, which was （ 1.077±0. 101 ） g/L, （ 10. 597±1. 283） g/L, （ 1. 272±0. 145） g/ L in healthy subjects respectively, there was a significant difference in the concentrations of Ig, A between two groups （ P 〈 0.05 ）, however there was no significant difference in IgG and lgM between two groups （ P 〉 0.05）. Conclusion There is T cell function imbalance in children with HSP at acute period, there is a imbalance between Thl and Th2, and Th2 cells are activated dominantly. The synthetic abnormality of plasma imrnunoglobulin lg, A is the important cause of producing immune complex angiitis.

关 键 词：过敏性紫癜 辅助性T淋巴细胞 CD4 CD30 儿童 免疫球蛋白 

分 类 号：R725.546[医药卫生—儿科] R392.11[医药卫生—临床医学]