胰升糖素与高脂血清对动脉平滑肌细胞形态学的影响  

The effects of different concentrations of glucagon with or without hyperlipidemic serum on the cytomorphology of smooth muscle cells

作　　者：刘铨之[1] 陈家伟[1] 沈捷[1] 蒋须勤[1] 张忠邦[1] 程宝庚[1] 薛婉芬[1] 邵亚男[1] 黄曼倩 

机构地区：[1]南京医科大学第一附院内分泌科

出　　处：《中国糖尿病杂志》1996年第2期94-97,共4页Chinese Journal of Diabetes

摘　　要：利用细胞培养技术研究胰升糖素浓度为１００、１０００以及１００００ｎｇ／Ｌ，在伴和不伴高脂血清条件下，各组兔主动脉平滑肌细胞之细胞形态学和细胞动力学变化。结果表明：①胰升糖素浓度为１００ｎｇ／Ｌ时，细胞形态学和细胞动力学均无明显变化。②胰升糖素浓度在１０００和１００００ｎｇ／Ｌ时，细胞轻度蜕变，ＤＮＡ增殖率呈抑制趋势；部分细胞内出现脂滴，后者有利于泡沫细胞形成。③高脂血清与胰升糖素之间对动脉粥样硬化形成有正的协同作用。本文结果提示：降低糖尿病患者胰升糖素和血脂水平，可延缓动脉粥样硬化的发生、发展。A study of the effects of glucagon in concentrations of 100,1000 and 10000 ng/L with or without hyperlipidemic serum(HLS)on the cytomorphology and cytodynamics of cultured rabbit arterial smooth muscle cells(SMC) was carried out by light microscopy,transmission electron microscopy and isotope technique,The results were as follows:①No effect of glucagon in concentration of 100 ng/L on SMC in both cytomorphology and cytodynamics was found.②Glucagon in concentration of 1000 or 10000 ng/L caused slight damage of SMC,and tended to inhibit the multiplication rate of DNA,a parameter of cytodynamics.Furthermore,lipidic substance in SMC was found under such concentrations of glucagon without HLS,which was favorable for formation of foam cells,③There was positively synergistic effect between glucagon and HLS in the development of atherosclerosis.Our results suggest that to keep lower levels of plasma glucagon and lipids in diabetic patients may delay the development of atherosclerosis.

关 键 词：胰升糖素 高脂血清 平滑肌细胞 动脉粥样硬化 

分 类 号：R543.502[医药卫生—心血管疾病]