微量元素对籼稻愈伤组织分化成苗的影响  

Trace elements influencing on seedlings differentiation from Indica rice calli

作　　者：崔广荣[1] 梁静[1] 张子学[1] 张从宇[1] 

机构地区：[1]安徽技术师范学院农学系,安徽凤阳233100

出　　处：《生物学杂志》2006年第1期22-25,共4页Journal of Biology

基　　金：安徽技术师范学院自然科学基金项目资助(ZRC200212)

摘　　要：通过研究MS培养基中碘、硼、锰、锌、钼、铜、钴以及铁8种微量无素对愈伤组织分化成苗的影响,结果表明:缺少碘、硼、锰、锌、钼、钴的培养基对籼稻愈伤组织分化成苗影响不大,而缺少铜和铁的培养基对籼稻愈伤组织分化成苗影响很大,添加不同浓度铁和铜元素的试验表明,在铁盐浓度为0.10mmo1.L-1,铜浓度为1.5×10-4mmo1.L-1时,分化成苗效果较好,而铁盐浓度分别在0.05mmol.L-1、0.15mmol.L-1、0.20mmol.L-1、0.40mmol.L-1,铜浓度分别在0.5×10-4mmol.L-1、1.0×10-4mmol.L-1、2.0×10-4mmol.L-1、4.0×10-4mmol.L-1时,对籼稻分化成苗和生长均不利。It was studied that the effects of I, B, Mn, Zn, Mo, Cu, Co and Fe on the calh and seedling differentiation from Indica rice. The results indicated that there were not remarkable effects on the calli and seedling differentiation when the media lacked of I, B, Mn, Zn, Mo. But when there was not enough content of Fe^2＋ and Cu^2＋ in the media, the effects were notable. When Fe2＋ with 0.10mmol L^-1 and Cu^2＋ with 1.5 × 10^-4mmol L^-1 ,the effects of seedling differentiation were better.

关 键 词：籼稻 愈伤组织 微量元素 分化成苗 

分 类 号：Q943.1[生物学—植物学]