HPLC法测定11种卷柏属药材中穗花杉双黄酮的含量  

Determination of Amentoflavone in 11 Species of Selaginella Medicinal Material by HPLC

作　　者：孙冬梅[1] 罗文汇[1] 李智勇[1] 

机构地区：[1]广东省中医研究所,广东广州510095

出　　处：《中药材》2006年第1期26-28,共3页Journal of Chinese Medicinal Materials

基　　金：广东省科委项目(A301020102)

摘　　要：采用HPLC法测定11种卷柏属药材中穗花杉双黄酮的含量。色谱柱：Kromasil（250×4．6mm，5μm）；流动相：甲醇旬．05mol／L磷酸二氢钠溶液系统，梯度洗脱，0～28min，甲醇50％～58％，30～35min，甲醇58％～70％；柱温：400C；流速：1．0ml／min；检测波长：330nm。结果表明，穗花杉双黄酮在153～767ng范围内峰面积呈良好的线性关系，平均回收率为97．03％，RSD为0．80％（n=5）。Objective： To determine the content of amentoflavone in 11 species of Selaginella medicinal materials. Methods： HPLC was used with kromasil （250 × 4. 6 mm,5 μm） column. The mobile phase consisted of methanol and phosphate buffer. The gradient condition was 0→28 min with methano1.50%→58%, and 30→35 min with methanol 58%→70%. The column temperature was 40℃. The flow rate was 1.0 ml/min and the detection wavelength at 330 nm. Results ： Amentoflavone showed a good linearity in the range of 153 ～ 767 ng. The average recovery was 97.03% and RSI） was 0. 80% （n = 5 ）. Conclusion： The method was rapid, accurate and can be used for quality control of Selaginella medicinal materials.

关 键 词：卷柏 穗花杉双黄酮 高效液相色谱法 

分 类 号：R282.71[医药卫生—中药学]