苦参碱逆转小鼠S_(180)肿瘤细胞获得性多药耐药基因相关表达产物过度表达的研究  

Study of Matrine′s Use on the Reversion of Obtained Multi-drug Resistance of Mice S_(180) Tumour Cell

作　　者：李贵海[1] 王玫[2] 孙付军[1] 王学荣[1] 李晓晶[1] 尹格平[3] 

机构地区：[1]山东省中医药研究院,山东济南250014 [2]山东省血液中心,山东济南250014 [3]济南军区总医院,山东济南250031

出　　处：《中药材》2006年第1期40-42,共3页Journal of Chinese Medicinal Materials

基　　金：山东省自然基金项目(Y2002C37)

摘　　要：目的:观察苦参碱对模拟临床化疗顺铂+5-氟尿嘧啶+环磷酰胺(PFC)方案诱导的小鼠S180细胞多药耐药相关基因表达产物过度表达的逆转作用,明确其对多药耐药逆转的作用及其作用的分子机制。方法:模拟临床PFC方案,分别给小鼠顺铂3 mg/kg.ip,环磷酰胺和5-氟尿嘧啶(5-FU)各3 mg/kg.ip,获得耐药S180小鼠模型。腹腔接种传代后24 h随机分组,对照组给予纯净水0.2 m l/10 g灌胃,苦参碱100 mg/kg和50 mg/kg组给分别给予0.5%、0.25%的苦参碱溶液0.2 m l/10 g灌胃,连续10天,流式细胞术免疫荧光检测肿瘤细胞多药耐药基因表达产物细胞膜糖粘蛋白(P170)、肺耐药蛋白(Lung resistance prote in,LRP)、DNA拓扑异构酶Ⅱ(DNA,Topoisom eraseⅡ,TOPOⅡ)的影响。结果:苦参碱降低耐药S180肿瘤多药耐药相关基因表达产物P170、LRP、TOPOⅡ表达率。结论:苦参碱逆转肿瘤多药耐药作用与其对肿瘤多种相关生物分子因子的调节有关。临床可以根据肿瘤多药耐药的性质,通过调节多药耐药相关因子逆转肿瘤化疗耐药性,提高临床化疗疗效。Objective：To Observe the effect caused by matrine＇s used on the reversion of obtained multi-drug resistance of mice S180＇s tumour cell induced by chemotherapy of Cisplatin ＋ 5-FU ＋ Cytoxan （PFC） and discuss its molecular mechanism. Methods： Patterned the methods of PFC chemotherapy in clinic, the mice were given Cisplatim 3 mg/kg·ip once a week and Cytoxan, CTX and 5-FU 3 mg/kg · ip once everyday for 4 weeks to set up the mice models of multi-drug resistance of S180 tumor cell. At the same time, gave the mice models matrine 4 weeks, and observed the P170, LRP, TOPO Ⅱ by flow cytometry. Results： matrine could obviously reduce the express of P170, LRP and the activiation of TOPO Ⅱ, correlated with mulit-drug resistance tumour cells which were induced by chemotherapy. Conclusion： Matrine, with its adjustment of correlated biotic active matter, can intervene the ocurrence of the multidrug resistance of tumor cells induced by chemotherapy.

关 键 词：苦参碱 肿瘤 小鼠 多药耐药 P170 LRP TOPO Ⅱ 

分 类 号：R285.5[医药卫生—中药学]