不同冷冻和解冻方法对小鼠桑椹胚发育的影响  

Effects of Different Cryopreservation and Dilution Methods on the Development of Mouse Morulae

作　　者：杨中强[1] 朱士恩[1] 周光斌[1] 侯云鹏[1] 闫长亮[1] 

机构地区：[1]中国农业大学动物科技学院动物胚胎生物技术实验室,北京100094

出　　处：《中国畜牧杂志》2006年第1期5-7,共3页Chinese Journal of Animal Science

基　　金：北京市农业科技重点项目(H022020060420)

摘　　要：本试验以2种程序化冷冻液和2种玻璃化冷冻液对昆明白系小鼠的桑椹胚进行细管法冷冻保存,比较程序化冷冻-管外解冻和玻璃化冷冻-管内解冻对胚胎体内、外发育的影响。胚胎体外培养结果表明:玻璃化冷冻组及程序化冷冻组胚胎发育率(95.3%￣95.8%,98.9%)无显著(P>0.05)差异。将程序化冷冻、EFS30玻璃化冷冻以及新鲜的胚胎各168枚移植给假孕受体鼠,妊娠受体产活仔率各组间相比(50.8%,58.3%,54.9%)无显著性(P>0.05)差异。结果证明,玻璃化冷冻保存的胚胎管内解冻效果好,为生产中家畜的胚胎移植提供了理论和技术参考。Two slow freezing solutions and two vitrification solutions were used in the present study to cryopreserve Kunming mouse morulae, which were loaded in plastic straws. Embryos after slow freezing were diluted out of the straws while embryos after vitrification were diluted in the straws, and the effects of different cryopreservation and dilution method on the development of embryo were compared. After cultured in vitro, differences on the developmental rates （95.3%-95.8%,98.9%） between vitrification and slow freezing with 1.5 mol/L ethylene glycol were not significant （P 〉 0.05）. Embryos after slow freezing and vitrification with EFS30 and fresh embryos were respectively transferred to recipients. Differences between the rates of embryos that develope to live fetuses of the pregnant mice were not significant （50.8%, 58.3%, 54.9%; P 〉 0.05）. In conclusion, in-thawing is efficient when used on embryos after vitrification. It can be used as a technical parameter in practice.

关 键 词：畜牧学 程序化 玻璃化 管内解冻 桑椹胚 小鼠 

分 类 号：S829.903[农业科学—畜牧学]