吡格列酮对人脐静脉内皮细胞CD40/CD40L表达的影响  

The effects of pioglitazone on the expression of CD40/CD40L in human umbilical vein endothelial cells

作　　者：楚罗湘[1] 姜德谦[1] 刘照云[1] 

机构地区：[1]中南大学湘雅二医院心内科,长沙410011

出　　处：《中国药物与临床》2006年第1期48-51,共4页Chinese Remedies & Clinics

摘　　要：目的探讨吡格列酮对氧化低密度脂蛋白(oxLDL)刺激下的人脐静脉内皮细胞(HUVECs)CD40/CD40L表达的影响。方法原代培养人脐静脉内皮细胞,给予oxLDL刺激和不同浓度吡格列酮干预。采用流式细胞技术检测CD40/CD40L在细胞上的表达,采用反转录-聚合酶链反应(RT-PCR)检测LOX-1mRNA的表达。结果OxLDL以浓度和时间依赖的方式刺激HUVECs表达CD40/CD40L,吡格列酮以剂量依赖的方式减轻ox-LDL刺激HUVECs表达CD40/CD40L。同时我们还观察到oxLDL能刺激HUVECs上调LOX-1mRNA的表达,而吡格列酮能明显抑制这种作用。结论吡格列酮能减轻oxLDL刺激下的人脐静脉内皮细胞CD40/CD40L的表达,其作用的机制可能与其抑制了HUVECs的LOX-1受体表达有关。Objective The CD40/CD40 Hgand pathway mediates inflammatory processes important in athemgenesis and the formation of the intraplaque lipid pooLIt was evaluated that the hypothesis pioglitazone decrease CD40/CD40L expression in human umbilical vein endothelial cells （HUVECs） induced by oxidized low-density lipoprotein （oxLDL）. Method HUVECs were incubated with oxLDL for different time periods and vary concentrations to determine the expression of CD40/CIMOL.HUVECs were also incubated with oxLDL （ 80 u g/ml ） for 24 hours with or without pretreated by pioglitazone.Expression of CD40/CD40L were detected by flow cytometric technology, and LOX-1 mRNA expression were evaluated by reverse transcription polymerase chain reaction （RT-PCR）.Results It was observed that ox-LDL increased the expression of CD40 and CD40L in a concentration-and time-dependent manner.Pioglitazone can decrese the upregulation of CD40/CD40L on HUVECs induced by ox-LDL. A high concentration of pioglitazone （10 umol/L） had a greater effect than the low concentration （1 u mol/L）.We also observed that ox-LDL upregulated LOX-1 mRNA expression on HU- VECs and pioglitazone can inhibited the effect in a dose-dependent manner, incubation of HUVECs with pioglitazone （10 u mol/L） alone did not affect CD40/CD40L and LOX-1 mRNA expression.Conclusion Lox-1 may play important roles in oxLDL uptake and subsequent functional alteration in the luminal endothelium in early atherosclerosis. Pioglitazone can inhibite the upregulation of LOX-1 in HUVECs elicited by oxLDL and subsequently decrease HUVECs CD40/CD40L expression induced by oxLDL These observation provide novel insigh into a potential novel antiinflammatory pathway of thiazolidinediones.

关 键 词：吡格列酮 内皮细胞 脐静脉 脂蛋白类 LDL 

分 类 号：R96[医药卫生—药理学]