粪肠球菌gyrA基因突变与氟喹诺酮类耐药性关系的研究  

Relationship between the mutation of gyrA gene in Enterococcus faecalis and the drug resistance of fluroquinolone

作　　者：刘志远[1] 许淑珍[1] 祁惠燕 

机构地区：[1]首都医科大学附属北京友谊医院,北京100050 [2]青海省西宁市第一人民医院

出　　处：《山东医药》2006年第4期17-18,共2页Shandong Medical Journal

基　　金：北京市自然科学基金资助项目(7012013)

摘　　要：目的探讨粪肠球菌DNA促旋酶A亚单位(gyrA)基因突变与氟喹诺酮类耐药的关系。方法琼脂稀释法测定粪肠球菌对各种氟喹诺酮类药物的耐药情况;聚合酶链反应(PCR)结合单链构象多态性(SSCP)分析检测gyrA中氟喹诺酮类耐药决定区(QRDR)突变情况。结果粪肠球菌对诺氟沙星耐药率为78.1%(50/64),加替沙星为54.7%(35/64)。41株耐药菌的第87位氨基酸编码碱基发生突变(GAA→GGA)。结论加替沙星的耐药率较低,优于其他氟喹诺酮类药物;gyrA基因突变是粪肠球菌对氟喹诺酮类耐药的重要原因。Objective ： To detect the mutation in fluroquinolone-resistant Enterococcus faecalis, and study the relationship between the resistance and mutation of gyrA. Methods： Obtained fluroquinolone-resistant Enterocoeeus faecalis by agar dilution method. The gyrA gene was amplified with PCR, and mutation was detected with SSCP and sequencing. Results： 78. 1% Enterococcus faecalis resisted to fluroquinolone, and 54.7% to gatifloxacin. Mutation was found in 41 strains of fluroquinolone-resistant Enterococcus faecalis, which was from Glu to Gly, and no mutation in other 9 strains. Conclusion： Gatifloxacin is better than other fluroquinolones. Mutation in gyrA is an important reason for fluroquinolone resistance. Other mechanisms maybe take part in the fluroquinolone resistance.

关 键 词：肠球菌 粪 氟喹诺酮 抗药性 基因 gyrA 

分 类 号：R378[医药卫生—病原生物学] R915[医药卫生—基础医学]