X射线照射对人树突状细胞免疫功能的影响  

EFFECT OF X-RAY ON IMMUNOLOGICAL FUNCTION OF HUMAN DENDRITIC CELLS

作　　者：吴明媛[1] 朱一蓓[1] 周菊英[2] 戴俊[1] 黄勇[1] 夏瑜[1] 强亦忠[1] 张学光[1] 

机构地区：[1]苏州大学生物技术研究所 [2]苏州大学附属第一医院放疗科,215006

出　　处：《辐射防护》2006年第1期35-39,共5页Radiation Protection

基　　金：国防科工委预研项目(Y5573162)

摘　　要：采用免疫荧光标记、流式细胞仪、3^H-TdR掺入和MTT技术，研究χ射线照射对人外周血来源的树突状细胞（DCs）和T细胞免疫功能的影响。结果显示，不同剂量（0．1～0．5Gy）χ射线照射后使DCs的HLA-DR、CD86和CD40分子表达上调，CD80分子表达下调；与其他剂量组相比，O．2Gy照射DCs可调节T细胞CD4／CD8比值，增强T细胞的增殖能力，同时还可提高特异性CTL细胞的杀伤能力。说明0．2Gyχ射线体外照射可一定程度提高受辐射损伤机体的免疫功能，是体外处理治疗性DCs较为合理的剂量。In this study, by means of immunofluorescence label, flow cytometer,3^H-TdR incorporation and MTI analysis, the immune function of dendritic cells（DCs） and T cells derived from normal human peripheral blood were investigated after X irradiation in different doses. The results show that the expressions of HLA-DR, CD86 and CD40 moleuculars of DCs were up-regulated after irradiation with different doses. DCs irradiated with 0.2 Gy could allow the restoration of CD4/CD8 proportion of T cells and the increase of T cell proliferation, as well as the cytotoxicity of CTL cells. It is suggested that DCs in vitro irradiated with 0.2 Gy could significantly improve the immune function and the 0.2 Gy is a reasonable dose in clinical therapy of DCs.

关 键 词：X射线 树突状细胞 免疫功能 

分 类 号：R392.1[医药卫生—免疫学]