猪囊虫DNA疫苗在猪体内的组织分布及其安全性探讨  

Tissue Distribution and Safety in Porcine Immunized with a DNA Vaccine of Taenia Solium Cysticercosis

作　　者：吴琳[1] 刁振宇[2] 邓小昭[2] 高健[2] 周宗安[2] 刘玉[2] 王元伦[2] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]南京军区军事医学研究所,江苏南京210002

出　　处：《药物生物技术》2006年第1期14-16,共3页Pharmaceutical Biotechnology

基　　金：江苏省"十五"攻关课题;项目号BG2000003

摘　　要：研究猪囊虫DNA疫苗pVAX-S-Δc-3n在仔猪体内的组织分布情况,并对其安全性进行分析。将猪囊虫DNA疫苗以肌注方式免疫仔猪,分别于接种后1 d,7 d,4 w,8 w,12 w分离各组织,提取组织总DNA,用PCR法扩增质粒上抗原基因的部分序列,分析质粒DNA在不同组织内的分布及随时间变化的情况。结果表明接种后第一天,除了脑,其余组织都能检测到质粒,但随时间延长,仅注射部位能检测到质粒,且数量不断下降。肌注质粒在猪体内注射部位以外的组织中较短时间就被清除,因此质粒与染色体发生整合的可能性不大。This paper is to explore the tissue distribution and safety in porcine immunized with a DNA vaccine named pVAX-S-△c-3n. The plasmid vaccine was intramuscularly injected into a new born porcine. After 1 d,7d,4w,8w, 12w, various tissues were removed from porcine, respectively. Total DNA was extracted. PCR was used to amplify the special sequence of concerned antigen gene included in the plasmid and to study the distribution and kinetics of the vaccine in various tissues. Results showed that plasmid was detected in all the tissues except brain 1 d after immunization. But with time going, plasmid was only found in injected sites, with quantity declining. Intramuscularly injected plasmid was cleared in short time except for sites of administration. There is little possibility for plasmid vaccine to integrate into the host genome.

关 键 词：猪囊虫病 DNA疫苗 组织分布 安全性 

分 类 号：R392.11[医药卫生—免疫学]