ABA对拟南芥los5-1突变体及其野生型保卫细胞质膜内向K^+通道的调节  

Abscisic Acid Regulation of inward K^+ Current in the Plasma Membrane of Guard Cells in Arabidopsis Wild-type and los5-1 mutant

作　　者：薛瑞丽[1] 周云[1] 安国勇[1] 江静[1] 吕东[1] 苗琛[1] 宋纯鹏[1] 

机构地区：[1]河南大学生命科学学院河南省植物逆境重点实验室,河南开封475001

出　　处：《四川大学学报（自然科学版）》2006年第1期190-195,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(2003CB114305);河南省高校杰出科研人才创新工程[2005KY(x010)];河南大学自然科学基金

摘　　要：以拟南芥(Arabidopsis thaliana)ABA缺失突变体los5-1及其相应野生型为材料,用细胞质膜钙离子通道的抑制剂LaCl3和细胞内钙离子的螯合剂EGTA处理,运用膜片钳技术探讨在ABA调节突变体和相应野生型保卫细胞质膜内向K+电流过程中Ca2+的作用.实验结果表明ABA均可以明显抑制拟南芥C24野生型及其突变体los5-1保卫细胞原生质体的内向K+电流,而且ABA对los5-1突变体及其相应野生型保卫细胞质膜内向K+通道的调节具有不同的Ca2+依赖性,说明ABA对突变体和野生型的K+通道的调节可能通过不同的信号转导机制.Using patch-clamp techniques, after the treatment of LaCl3 （inhibitor of Ca^2 ＋ channels in the plasma membrane of guard cells ）and EGTA（chelator of cytosolic Ca^2＋ ）, the effects of Ca^2＋ on inward K^＋ channel activity which regulated by ABA in the plasma membrane of guard cells from Arabidopsis thaliana （wild-type C44 and ABA deficient mutant los5- 1 ） were examined. The results showed that inward currents in the guard cells of wild-type and mutant were significantly decreased within 20 mins after 10μmol/L ABA was perfused to the bath solution, and the Ca^2＋ dependence of abscisic acid regulation of guard cell inward K^＋ currents in Arabidopsis wild-type and los 5-1 mutant is different, this suggested that ahsdsic acid regulation of guard cell inward K^＋ current was mediated by different transduction pathways in Arabidopsis wild-type and los5-1 mutant.

关 键 词：膜片钳 内向K^+ 电流 ABA CA^2+ 

分 类 号：Q942[生物学—植物学]