卡托普利对血管平滑肌细胞内pH影响  

Effect of captopril on intracellular pH in vascular smooth muscle cells 1

作　　者：齐建华[1,2,3] 章鲁[1,2,3] 王军 顾培坤[1,2,3] 金正均 黄明智[1,2,3] 王新明[4] 

机构地区：[1]上海第二医科大学药理教研室 [2]计算机医学应用教研室 [3]上海市高血压研究所 [4]中国科学院上海细胞生物学研究所

出　　处：《中国药理学报》1996年第5期448-450,共3页Acta Pharmacologica Sinica

摘　　要：卡托普利对血管平滑肌细胞内ｐＨ影响１齐建华，章鲁２，王军，顾培坤，金正均，黄明智３（上海第二医科大学药理教研室，２计算机医学应用教研室，３上海市高血压研究所，上海２０００２５，中国）王新明（中国科学院上海细胞生物学研究所，上海２０００３１，中国）关键...IM: To observe the effect of captopril (Cap) on intracellular pH (pH i) in aortic smooth muscle cells (ASMC). METHODS: Cultured ASMC derived from rat and rabbit aortae were loaded with the fluorescent dye BCECF and pH i was determined using digital image processing method. RESULTS: The pH i of untreated SHR and WKY rats were 7 37±0 29 and 7 19±0 31, respec￣tively. Oral Cap decreased pH i (7 11±0 26, P <0 05) and exaggerated pH i response to angiotensin Ⅱ (Ang Ⅱ, 0 1 μmol·L -1 ) in ASMC of SHR rats vs WKY rats (0 14±0 05 vs 0 21 ±0 05 pH units, P <0 01). Cap in vitro had no effect on Ang Ⅱ induced intracellular alkalinization in ASMC of rabbits. CONCLUSION: Oral Cap inhibits Na +-H + exchange activity in ASMC of SHR rats.

关 键 词：胸主动脉 平滑骨 PH值 血管紧张素Ⅱ 卡托普利 

分 类 号：R972[医药卫生—药品] R962[医药卫生—药学]