厚荚相思树根瘤菌HJ06菌株的16SrDNA全序列和nifA基因片段分析  

16S rDNA and nifA Gene Sequence Analysis of Rhizobium Strain HJ06 Isolated from Acacia crassicarpa

作　　者：吕成群[1] 黄宝灵[1] 庄培亮[1] 武波[2] 

机构地区：[1]广西大学林学院,南宁530005 [2]广西大学生命科学与技术学院,南宁530005

出　　处：《热带亚热带植物学报》2006年第1期19-24,共6页Journal of Tropical and Subtropical Botany

基　　金：广西科学基金项目(桂科基0448005);广西"十五"林业科学研究项目(林科字2002第19号)资助

摘　　要：对厚荚相思(Acaciacrassicarpa)根瘤菌HJ06菌株的16SrDNA全序列和nifA基因片段进行了测定。结果表明,HJ06菌株在以16SrDNA序列构建的系统发育树状图中位于根瘤菌属(Rhizobium)分支中,与根瘤菌属各个种的相似性达95%以上;从HJ06菌株克隆出的585bpnifA基因片段与Klebsiellapneumoniae的同源性达到99.3%,与Klebsiellaoxytoca的NifF,NifL,NifA,NifB蛋白基因的同源性为97.8%。The determination of full-length 16S rDNA sequence and nifA gene segments of root nodule bacterium strain HJ06 isolated from Acacia crassicarpa showed that strain HJ06 had above 95% similarity with the genus Rhizobium. The nifA gene segments of HJ06 have 99.3% isogenesis with nifA genes of Klebsiella pneumoniae and 97.8% isogenesis with genes ofNifF, NifL, NifA and NifB proteins of K. oxytoca.

关 键 词：厚荚相思 根瘤菌 16S RDNA 全序列 系统发育 NIFA基因 

分 类 号：S718.81[农业科学—林学]