心脑肾康对肾缺血再灌注损伤模型大鼠的保护作用研究  

Research on Protection of Xinnaoshenkang(XNSK)on Renal Ischemical Reperfusion Injury Model Rats

作　　者：简洁[1] 李春凤[2] 梁钢[3] 徐庆[1] 李学孝[4] 

机构地区：[1]桂林医学院药理教研室,桂林市541004 [2]桂林医学院附属医院医务部,桂林市541001 [3]广西医科大学药理教研室,南宁市530021 [4]桂林医学院,桂林市541004

出　　处：《中国药房》2006年第4期260-262,共3页China Pharmacy

基　　金：广西科技厅科研基金资助课题(桂科攻0235024-3)

摘　　要：目的:探讨心脑肾康对肾缺血再灌注损伤模型大鼠的保护作用。方法:将60只大鼠随机分为假手术组(不夹闭双肾动脉、0·1%二甲基亚砜生理盐水)、对照组(0·1%二甲基亚砜生理盐水)、阳性对照组(肾复康胶囊)以及心脑肾康低、中、高剂量组,分别测定血清肌酐(Scr)、尿素氮(BUN)及肾组织内丙二醛(MDA)、超氧化物歧化酶(SOD)、活性氧(ROS)、一氧化氮(NO)的含量,并观察组织病理形态学改变。结果:与对照组比较,3个剂量的心脑肾康组于术前给药7d、术后再灌注1次,均能使肾缺血再灌注损伤模型大鼠中Scr、BUN、MDA、ROS、NO含量降低,SOD含量升高;可减轻肾组织病理形态学改变(P<0·05),且呈剂量依赖性。其中,高剂量组效果最好,优于阳性对照组(P<0·05)。结论:心脑肾康对肾缺血再灌注损伤模型大鼠具有保护作用,其机制可能与改善肾功能、抗自由基损伤有关。OBJECTIVE： To study the protection of XNSK on renal ischemical reperfusion injury model rats. METHODS： A total of 60 rats were randomized into sham operation group （without occlusion of renal arteries of both kidneys and administered with 0.1% dimethyl sulfoxide normal saline）, control group（0.1% dimethyl sulfoxide normal saline）, positive control group （administered with Shenfukang capsules） and XNSK （high, mcdium and low dose subgroups） group. Levels of serum creatinine （Scr）, urea nitrogen （BUN） and malonaldehyde （MDA） in renal tissue, hemocuprein （SOD）, active oxygen （ROS） and nitrogen monoxidum（NO） were determined respectively meanwhile the pathomorphology changes of tissues were observed. RESULTS： As compared with the control group, levels of Scr, BUN, MDA, ROS and NO in all the 3（high, medium and low doses） XNSK subgroups （which were administered with drugs for 7 days before operation and undergone reperfusion once after operation） had a reduction while SOI） level had an increase. And there was a mitigated pathomorphology change in nephridial tissues（P〈0.05）, and which was dose dependent. The high dose subgroup was of the best efficacy , superior to positive control group（P〈 0.05） . CONCLUSION： XNSK has protective effect on renal ischemical reperfusion injury model rats. The mechanism is possibly related to amelioration of renal function and against injury of free radicals.

关 键 词：心脑 肾康 净缺血再灌注损伤 大鼠 保护作用 

分 类 号：R965[医药卫生—药理学]