含马兜铃酸类中药材中马兜铃总酸的含量  

Determination of Total Aristolochic Acid in Traditional Chinese Medicines Containing Aristolochic Acid By Ultraviolet Spectrophotometry

作　　者：李琳[1] 王智民[1] 高慧敏[1] 王冠[2] 何希荣[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中国实验方剂学杂志》2006年第2期11-13,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金资助项目(No:30271612)

摘　　要：目的：建立马兜铃总酸的含量测定方法，并测定不同地区市售青术香及其它八种含马兜铃酸类中药中马兜铃总酸的含量，用于指导临床安全、合理用药。方法：用紫外分光光度法分析，检测波长为390nm。结果：马兜铃酸A在0．047。0．235mg范围内（r=0．9992）有良好的线性关系；平均加样回收率为98．16％，RSD=2．04％。不同地区市售青木香中马兜铃总酸含量在0．145％～0．567％范围内，其中以河北安国含量最高（0．567％），重庆含量最低（0．145％）；八种含马兜铃酸类中药材中马兜铃总酸含量在0．178％～0．717％范围内，其中以关木通含量最高（0．717％），华细辛含量最低（0．178％）。结论：本方法简便、准确，可用来测定其它含有马兜铃酸类中药材及中成药中马兜铃总酸的含量。Objective：To develop a determination method of total aristolochic acid by ultraviolet spectrophotometry to ensure cliriical safety and therapeutic efficacy. Methods： The samples were analyzed by ultraviolet spectrophotometry and detected at wave length of 390nm. Results：The calibration curve was linear （r = 0,9992） within the range of 0.047 0. 235mg for arlstolochic acid A. The average recovery was 98.16 %. RSD = 2.04 %, Conclusion ： The method with good linear relationship was convenient, quick, accurate, and suitable for the quality control of the total aristolochic acid in traditional Chinese medicines containing aristolochic acid.

关 键 词：马兜铃总酸 紫外分光光度法 含量测定 

分 类 号：R284.1[医药卫生—中药学]