气相色谱法测定动物组织中氯霉素的残留量  

Determination of Chloramphenicol Residue in Animal Tissues by GC

作　　者：洪振涛[1] 张卫锋[1] 聂建荣[1] 朱孟丽 庄志辉[1] 

机构地区：[1]广州市农业标准与监测中心,广东广州510315

出　　处：《中国兽药杂志》2006年第2期14-16,共3页Chinese Journal of Veterinary Drug

摘　　要：采用气相色谱法测定鸡肉、猪肉、牛肉、猪肝、虾、鱼等动物组织样品中氯霉素的残留量，对行业标准进行部分改进，使方法检出限降低了2个数量级，扩大了方法适用范围，同时简化了样品前处理。氯霉素浓度在1．00-500μg／L范围内，衍生物的峰面积与氯霉素浓度呈良好的线性关系，回归方程为y=121．96x-212．06，相关系数大于0．9999。添加回收率在85．3％-103．8％之间，变异系数小于5．32％。对氯霉素的检出限低于0．1μg／kg，定量限为0．3μg/kg。GC determination method of chloramphenicol residue in animal tissue （ chick, pork,beef, pig liver, shrimp fish）was modified, the sample preparation became very simple and efficient. There was a good linear correlation （ the coefficient of correlation was above 0.999 9 ） between the peak areas and concentration of chloramphenicol with the range from 1.00 to 500 μg/L. The regression equation was y = 121.96 x - 212.06. The average recoveries of chloramphenicol fortified in animal tissues were 85.3 % - 103.8 % with the relative standard deviation below 5.32 % and the detection limit was below 0.1μg/kg,the quautitative limit was

关 键 词：气相色谱法 动物组织 氯霉素 

分 类 号：S859.84[农业科学—临床兽医学]