丙体六六六对斜生栅藻生长及光合色素和膜脂过氧化影响的研究  

Effects of γ-HCH on the Growth and Photosystem and Lipid Peroxidation of Scenedesmus Obliqnus kütz

作　　者：刘碧云[1] 周培疆[1] 李佳洁[1] 吴兆录[2] 宋立荣[3] 

机构地区：[1]武汉大学资源与环境科学学院,湖北武汉430079 [2]云南大学生态学与地植物学研究所,云南昆明650091 [3]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072

出　　处：《农业环境科学学报》2006年第1期204-207,共4页Journal of Agro-Environment Science

基　　金：国家重点基础研究发展规划项目(2002CB4123061);国家自然科学基金(20177018);国家自然科学基金(20577036)

摘　　要：采用室内培养试验方法,研究了丙体六六六(γ-HCH)对斜生栅藻生长和生理生化的影响。结果表明,在γ-HCH浓度<0.2mg·L-1时对斜生栅藻生长有促进作用,在2mg·L-1时则表现为抑制作用,其对叶绿素的影响与对藻密度的影响一致,显示浓度-效应关系。γ-HCH对斜生栅藻的超氧化物歧化酶(SOD)的影响表现为,在0.02mg·L-1时达到峰值,然后下降,在2mg·L-1时其活性只有对照的38.18%。γ-HCH对斜生栅藻的膜脂过氧化的影响,在<0.02mg·L-1时变化不大,而在0.2mg·L-1和2mg·L-1时有一定的升高;MDA的变化与SOD的变化有一定的相关性,在SOD急剧下降时,MDA的含量上升较快。Gamma hexaehlorocyclohexane （γ-HCH） is one of the most important compositions of hexachloroeyclohexane （HCH）, organochlorine pesticides. The organochlorine pesticides have severe physiological effects on organisms, including human. The effect of γ-HCH on the green algae, Scenedesmus Obliqnus kütz, was examined. The results showed that the growth, chlorophyll contents, SOD activity of Scenedesmus Obliqnus ktttz were stimulated under low γ-HCH concentration （0.2 mg·L^-1） and inhibited under high γ-HCH concentration （2 mg·L^-1）. The SOD activity reached the peak of the curve in 0.02 mg·L^-1 and reduced to 38.18% in 2 mg·L^-1 2 of γ-HCH culture medium. Correspondingly, MDA contents showed no significant changes in Scenedesmus Obliqnus kütz treated with 0.002 mg·L^-1 and 0.02 mg·L^-1 of γ-HCH, but increased by 38.6% and 158.8% when treated with 0.2mg·L^-1 and 2 mg·L^-1 of γ-HCH, compared with the untreated samples. These results implied that high γ-HCH concentration might have adverse effect on Scenedesmus Obliqnus kütz.

关 键 词：斜生栅藻 生长动力学 超氧化物歧化酶(SOD) 丙二醛(MDA) 丙体六六六 

分 类 号：X592[环境科学与工程—环境工程]