系统性红斑狼疮患者白介素10受体cDNA基因多态性研究  

Polymorphism within the interleukin-10 receptor cDNA gene in patients with systemic lupus erythematosus

作　　者：姚煦[1] 李遇梅[1] 胡兹嘉[1] 李文忠[1] 陈志强[1] 

机构地区：[1]中国医学科学院,中国协和医科大学皮肤病研究所,南京210042

出　　处：《中华皮肤科杂志》2006年第1期7-9,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目(30170863)

摘　　要：目的探讨白介素10受体(IL-10R)的两个基因位点G241A和G520A基因多态性与系统性红斑狼疮(SLE)患者发病的相关性。方法采用聚合酶链反应-单链构象多态性(PCR-SSCP)和聚合酶链反应限制性片段多态性(PCR-RFLP)及DNA测序方法进行基因分型。结果IL-10R2基因型与SLE易感性显著相关。G520/G520基因型增加SLE发病危险性,P=0.004,OR=0.515,95%的可信区间为0.444-0.579;G520/A520基因型增加SLE发病危险性,P=0.055,OR=1.968,95%的可信区间为0.981-3.949。IL-10R1基因型与SLE易感性无显著相关。各个基因型组合的分布情况和优势比显示具有IL-10R1G241/G241基因型和IL-10R2G520/G520的个体SLE发病危险性明显高于其他的基因型组合,P=0.004,OR=0.515,95%的可信区间为0.444-0.597。结论IL-10R2基因型与SLE易感性显著相关,G520/G520基因型增加SLE发病危险性。具有IL-10R1G241/G241基因型和IL-10R2G520/G520的个体SLE发病危险性明显高于其他的基因型组合。Objective To assess the association between polymorphism within the interleukin-10 receptor cDNA gene （IL-10R） and Chinese patients with systemic lupus erythematosus （SLE）. Methods The IL-10R genotypes of 94 SLE patients and 80 healthy subjects were examined by reverse transcription-polymerase chain reaction-single strand conformation polymorphism method （RT-PCR-SSCP）, RT-PCR-restriction fragment length polymorphism （ RFLP ） and DNA sequencing. Results There were significant differences in the IL-10R2 genotype fi＇equencies of these two groups. The IL-10R2 G520/G520 genotype increased the risk of developing SLE （OR = 0.515, 95% CI 0.414-0.579, P = 0.004） and individuals who had G520/A520 genotype also had a higher susceptibility to SLE （OR = 1.968, 95% CI 0.981-3.949, P = 0.055）. There was no significant association between SLE and IL-10R1 genotypes. The risk of developing SLE was detected in the individuals who had the combination of IL-10R1 G241/G241 and IL-10R2 G520/G520 （OR = 0.515, 95% CI 0.444-0.597, P = 0.004）. Conclusion The IL-10R2 genotypes of G520/G520 and G520/A520 as well as the combination of genotypes IL-10R1G241/G241 and IL-10R2 G520/G520 may increase the susceptibility to SLE in Chinese people.

关 键 词：红斑狼疮 系统性 受体 白细胞介素 多态性现象 单链构象 

分 类 号：R593.241[医药卫生—内科学]