人源抗D-二聚体抗体的构建和分析  

Construction and characterization of hominine anti-D-dimer antibody

作　　者：陈颖[1] 田朝伟[2] 杨晓仪[1] 贾宗剑[1] 许健[1] 黄少华[2] 陈敏生[2] 吴文言[1] 

机构地区：[1]中山大学生命科学学院生物防治国家重点实验室,广州510275 [2]广州医学院第二附属医院,广州510260

出　　处：《免疫学杂志》2006年第1期34-36,39,共4页Immunological Journal

基　　金：广州市重点科技攻关资助项目(1999-X-006-01)

摘　　要：目的得到具有抗D-二聚体活性的人源抗体。方法应用噬菌体展示技术构建血栓病人的抗体库,展示了血栓病人的抗体,从中筛选出能与血栓主要成分纤维蛋白降解产物D-二聚体有特异结合作用的抗体。将抗体在大肠杆菌XL1-Blue中表达。再利用抗D-二聚体鼠源抗体进行竞争ELISA检验,证实抗体与D-二聚体结合的活性。结果从血栓病人抗体库中得到抗体p13株,并得到可溶表达,在ELISA检测中显示与D-二聚体结合的优势。结论该抗体株p13可供进一步开展导向溶栓剂的研究。Objective To pick out a human antibody that could interacted with D-dimer. Methods A human phage-displayed Fab library was constructed by using phage surface display technology. The antibodies, which could interact with D-dimer, were obtained by biopanning, and then expressed in XL1-Blue. The D-dimer-binding activity of the antibodies was assured by a competition ELISA test. Results The antibody numbered p13 was solubly expressed in XL1-Blue, and had a higher binding activity to D-dimer proved by ELISA. Conclusion The success of isolating human anti-D-dimer Fab p13 provides a base for further study of targeted thrombolytic.

关 键 词：人源抗D-二聚体抗体 噬菌体展示 导向溶栓 

分 类 号：R392[医药卫生—免疫学]