表达新城疫病毒F基因重组鸡痘病毒的筛选  

Selection and Protective Efficacy of Recombinant Fowlpox Virus Vaccine Expressing Fusion Protein Gene of Newcastle Disease Virus

作　　者：张体银[1] 孙蕾[1] 梁萍[1] 刘秀梵[1] 

机构地区：[1]扬州大学农业部畜禽传染病学重点开放实验室,江苏扬州225009

出　　处：《中国兽医学报》2006年第1期20-23,共4页Chinese Journal of Veterinary Science

基　　金：国家"863"计划资助项目(2001AA213041);科技部农业科技成果转化资金资助项目(OIEFN213201078)

摘　　要：将转移载体P11SF和PN11SF分别与282E4株鸡痘病毒(282E4strain fowlpox virus,282E4FPV)和大空斑株鸡痘病毒(large plaque strain fowlpox virus.LP FPV)共转染鸡胚成纤维细胞,通过蓝斑筛选,得到4株重组鸡痘病毒rFPV282E4-SFA、rFPVLP-SFA、rFPV282E4-SFB和rFPVLP-SFB,通过PCR和间接免疫荧光鉴定均为阳性,另外将各重组病毒分别传20代,测其遗传稳定性,结果显示均具有良好的稳定性。对SPF鸡进行免疫效力试验,攻毒后均100%保护,而对于商品鸡,保护率分别为64%、60%、52%和88%。The transfer vectors P11SF and PN11SF were transfected on CEF infected with parent 282E4 and large plaque strain fowlpox virus and four rFPVs（rFPV282E4-SFA,rFPVLP-SFx ,rFPV282E4-SFB,rFPVLP-SFB） were selected by blue plaques expressing β-galactosidase. The insertion and expression of F genes in rFPVs were confirmed by PCR and IFA respectively. They were serially passaged to the 20 th on CEF monolayers to evaluate their genetic stability. The results showed that they were all genetically stable for at least 20 passages. SPF chickens vaccinated with the rFPVs were challenged with 10^5ELD50 of F48E8 strain NDV.and all chickens were survived. While commercial chickens immunized with the four rFPVs respectively were challenged with Fd8E8 strain NDV.with the protection rates of 64 % ,60% ,52% .and 88% respectively.

关 键 词：新城疫病毒 F基因 重组鸡痘病毒 筛选 免疫效力 

分 类 号：Q78[生物学—分子生物学] S852.65[农业科学—基础兽医学]