PCR-SSCP对我国鲁道夫对盲囊线虫的分子鉴定  

Molecular Identification of Contracaecum rudolphii from China by PCR-SSCP

作　　者：陈虹虹[1] 陈晓梅[1] 杨廷宝[2] 何芳[1] 翁亚彪[1] 林瑞庆[1] 朱兴全[1] 

机构地区：[1]华南农业大学兽医学院 [2]中山大学生命科学学院

出　　处：《中国兽医学报》2006年第1期35-38,共4页Chinese Journal of Veterinary Science

基　　金：国家杰出青年科学基金资助项目(30225033)

摘　　要：对来自青海湖的鲁道夫对盲囊线虫(Contracaecum rudolphii)核糖体DNA第一、第二内转录间隔区(ITS-1、ITS-2)进行PCR扩增、DNA单链构象多态性(SSCP)分析及序列分析,并与来自欧洲的2个姊妹种鲁道夫对盲囊线虫进行了比较。结果显示,我国青海湖的鲁道夫对盲囊线虫与来自意大利的C.rudolphii B具有一样的SSCP带型及ITS序列,但不同于C.rudolphii A,因此属于C.rudolphii B。本试验证实了ITS片段可作为遗传标记用于鉴别鲁道夫对盲囊线虫的姊妹种,从而为鲁道夫对盲囊线虫的进一步研究奠定了基础。The aim of the present study was to identify specimens representing Contrancaecum rudolphii which were isolated from Qinghai Lake,China. The first and the second internal transcribed spacers（ITS-1 ,ITS-2） of ribosomal DNA were amplified by PCR from individual Chinese C. rudolphii and the amplicons were analysed by single-strand conformation polymorphism （SSCP）. The results of SSCP analyses revealed that all the Chinese C. rudolphii specimens represented C. rudophii B. One specimen was sequenced and the ITS-1 and ITS-2 sequences were identical to that of C. rudophii B,consistent with the results of SSCP analysis. For the first time in China,this study demonstrated that ITS-1 and ITS-2 sequences could be applied as genetic markers for the identification of Chinese C. rudolphii,and the findings have implications for studying the systematics and molecular genetics of anisakid nematodes.

关 键 词：鲁道夫对盲囊线虫 内转录间隔区(ITS) PCR-SSCP 序列分析 

分 类 号：Q78[生物学—分子生物学] S852.73[农业科学—基础兽医学]