小偃6号抗条锈基因遗传分析及分子标记  

Genetic Analysis and Molecular Markers of the Resistance Genes of Xiaoyan 6 to Stripe Rust

作　　者：孔凡娜[1] 徐智斌[1] 姚秋燕[1] 王美南[1] 井金学[1] 商鸿生[1] 李振岐[1] 

机构地区：[1]西北农林科技大学植物保护学院

出　　处：《西北植物学报》2006年第1期23-27,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家"十五"攻关课题(2001BA509B503);"973"项目(G2000016202);西北农林科技大学创新团队资助项目

摘　　要：用小麦条锈菌CY29-mut3、CY28、CY27和CY25分别接种小偃6号、铭贤169及其F2代各株系，在常温下（15～17℃）和高温下（20～22℃）进行了小偃6号抗条锈基因的遗传分析。结果发现，在常温下，小偃6号对4个条锈菌生理小种的抗病性均由1对显性核基因控制；在高温下，其抗病性由2对或3对基因控制，但其正反交的作用方式不同，抗锈性也可能与细胞质遗传有关；筛选到与抗条锈基因连锁的RAPD标记，分别命名为OPT17650、OPC111000。同时，具有长穗偃麦草血缘的小麦品种小偃22对OPC11进行了验证，明确了其在分子辅助育种中的价值。Xiaoyan 6 and Mingxian 169 and their F2 lines were inoculated with the races CY29-mut3,CY28, CY25 of Puccinia striiformis f. sp. tritici and the resistance gene of Xiaoyan 6 to Puccinia striiformis f. sp. tritici were genetically analyzed at normal temperature （15-17℃） and high temperature （20-22℃）. The results showed that a pair of dominant genes controlled the resistances of the four varieties and lines to the races at normal temperature two or three pairs of genes control the resistances at high temperature, but their action mechanism differed between direct and reciprocal crosses,which indicated that the resistances to stripe rust had something to do with cytoplasmic inheritance;two RAPD markers linked to the resistance genes were screened and named OPT17650 and OPC111000. Meantime,the performance of OPTC-11 was verified with Xiaoyan 22,a cultivar heaving blood from long-spike oat grass so that its importance was confirmed in molecularly-aided breeding.

关 键 词：小麦 条锈菌 抗条锈性遗传 RAPD 

分 类 号：S512.1[农业科学—作物学] S435.121.4