盐胁迫下盐地碱蓬叶片液泡膜H^+-ATPase H亚基的克隆与表达分析  

Cloning and Expression of Subunit H of V-H^+-ATPase in Vacuole Membrane in the Leaves of the Halophyte Suaeda salsa Under Salt Stress

作　　者：李艳艳[1] 李平华[1] 王宝山[1] 

机构地区：[1]山东师范大学生命科学学院,济南250014

出　　处：《西北植物学报》2006年第1期63-67,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30270793);山东省自然科学基金重点项目(Z2004D03)

摘　　要：利用EST随机挑取克隆测序的方法从盐地碱蓬叶片cDNA文库中分离得到了盐地碱蓬V-H+-ATPase H亚基cDNA序列,并进行了H、c亚基基因表达及V-H+-ATPase活性分析.结果表明,H亚基基因全长1 969 bp,包括100 bp的5′-非编码区和471 bp的3′-非编码区.开放阅读框为1 398 bp,编码465个氨基酸残基,分子量约52.8 kD.N orthern杂交分析表明盐胁迫明显诱导了H亚基表达,而且盐胁迫下H、c亚基及V-H+-ATPase活性存在协同作用.这些结果表明盐胁迫下H和c亚基基因上调及V-H+-ATPase活性的增加为N a+区隔化到液泡中提供了质子驱动力.The cDNA sequence of subunit H of V-H^＋-ATPase was isolated by EST random cloning and sequencing from cDNA library of Suaeda salsa leaves and then the expressions of ssVHA-H and ssVHA-c and the activity of V-H^＋-ATPase were analyzed. The results showed that the full length of H-subunit was 1 969 bp,including 100 bp 5＇-noncoding region and 471 bp 3＇-coding region. The open reading frame was 1398 bp,encoding 465 amino acid residues and having a molecular weight of 52.8 kD. Salt stress was found capable of significantly inducing subunit H expression,and ssVHA-H,and ssVHA-c and the activity of V-H^＋-ATPase appeared to act in a synergistic manner under the stress. These indicated that under salt stress the expression up-regulations of ssVHA-H and ssVHA-c genes and the increase of the activity of V-H^＋-ATPase provide the proton driving force for sequestrating Na^＋ in vacuoles.

关 键 词：H亚基 C亚基 V-H^+ -ATPase 盐地碱蓬 盐胁迫 

分 类 号：Q945.78[生物学—植物学]